Müller M, Hammann S, Vetter W (2018)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2018
Publisher: Elsevier Ltd
Book Volume: 256
Pages Range: 327-332
URI: https://www.sciencedirect.com/science/article/pii/S0308814618303819
DOI: 10.1016/j.foodchem.2018.02.133
A new vitamin E homologue, α-tocomonoenol was detected in palm oil, but was not isolated in large amounts and with high purity so far. Here we present an easy and fast method to isolate α-tocomonoenol from vitamin E rich nutrient capsules with countercurrent chromatography (CCC). With the solvent system n-hexane – benzotrifluoride – acetonitrile (10:3.5:6.5, v/v/v) about 30 mg α-tocomonoenol with a purity of 75% could be enriched in one step from 1 g crude sample. Column chromatography with 20% deactivated silica gel and n-hexane – ethyl acetate (95:5, v/v) was performed to gain 5.6 mg α-tocomonoenol with a purity of 99.5% according to GC/MS. Structural verification by 1H NMR spectroscopy verified that the double bond was located in 11′-position (11′-α-tocomonoenol). The trace impurity detected in the isolate was identified to be 12′-α-tocomonoenol, a compound previously detected in marine samples.
APA:
Müller, M., Hammann, S., & Vetter, W. (2018). Countercurrent chromatographic isolation and purification of 11′-α-tocomonoenol from the vitamin E extract of palm oil. Food Chemistry, 256, 327-332. https://doi.org/10.1016/j.foodchem.2018.02.133
MLA:
Müller, Marco, Simon Hammann, and Walter Vetter. "Countercurrent chromatographic isolation and purification of 11′-α-tocomonoenol from the vitamin E extract of palm oil." Food Chemistry 256 (2018): 327-332.
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