SPIONs functionalized with small peptides for binding of lipopolysaccharide, a pathophysiologically relevant microbial product.

Karawacka W, Janko C, Unterweger H, Mühlberger M, Lyer S, Taccardi N, Mokhir A, Jira W, Peukert W, Boccaccini AR, Kolot M, Strauss R, Bogdan C, Alexiou C, Tietze R (2018)

Publication Status: Published

Publication Type: Journal article

Publication year: 2018

Journal

Colloids and Surfaces B-Biointerfaces Elsevier

Book Volume: 174

Pages Range: 95-102

DOI: 10.1016/j.colsurfb.2018.11.002

Abstract

Systemic inflammation such as sepsis represents an acute life-threatening condition, to which often no timely remedy can be found. A promising strategy may be to functionalize magnetic nanoparticles with specific peptides, derived from the binding motives of agglutinating salivary proteins, that allow immobilization of pathogens. In this work, superparamagnetic iron oxide nanoparticles with stable polycondensed aminoalkylsilane layer were developed, to which the heterobifunctional linkers N-succinimidyl 3-(2-pyridyldithio)-propanoate (SDPD) and N-succinimidyl bromoacetate (SBA) were bound. These linkers were further chemoselectively reacted with the thiol group of singularly present cysteines of selected peptides. The resulting functional nanoparticles underwent a detailed physicochemical characterization. The biocompatibility of the primarily coated aminoalkylsilane particles was also investigated. To test the pathogen-binding efficacy of the particles, the lipopolysaccharide-immobilization capacity of the peptide-coated particles was compared with free peptides. Here, one particle-bound peptide species succeeded in capturing 90% of the toxin, whereas the degree of immobilization of the toxin with a system that varied in the sequence of the peptide dropped to 35%. With these promising results, we hope to develop extracorporeal magnetic clearance systems for removing pathogens from the human body in order to accelerate diagnosis and alleviate acute disease conditions such as sepsis.

Authors with CRIS profile

Christina Janko Department of Otorhinolaryngology – Head and Neck Surgery Harald Unterweger Department of Otorhinolaryngology – Head and Neck Surgery Marina Mühlberger Department of Otorhinolaryngology – Head and Neck Surgery Stefan Lyer Department of Otorhinolaryngology – Head and Neck Surgery Nicola Taccardi Lehrstuhl für Chemische Reaktionstechnik Andriy Mokhir Professur für Organische Chemie Wolfgang Peukert Lehrstuhl für Partikelsynthese Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Christian Bogdan Lehrstuhl für Mikrobiologie und Infektionsimmunologie Christoph Alexiou Professur für Nanomedizin Rainer Tietze Department of Otorhinolaryngology – Head and Neck Surgery

Involved external institutions

Tel Aviv University IL Israel (IL) Max-Rubner-Institut - Bundesforschungsinstitut für Ernährung und Lebensmittel DE Germany (DE)

How to cite

APA:

Karawacka, W., Janko, C., Unterweger, H., Mühlberger, M., Lyer, S., Taccardi, N.,... Tietze, R. (2018). SPIONs functionalized with small peptides for binding of lipopolysaccharide, a pathophysiologically relevant microbial product. Colloids and Surfaces B-Biointerfaces, 174, 95-102. https://doi.org/10.1016/j.colsurfb.2018.11.002

MLA:

Karawacka, Weronika, et al. "SPIONs functionalized with small peptides for binding of lipopolysaccharide, a pathophysiologically relevant microbial product." Colloids and Surfaces B-Biointerfaces 174 (2018): 95-102.

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