Journal article


Comprehensive analysis of nonenzymatic post-translational beta-lactoglobulin modifications in processed milk by ultrahigh-performance liquid chromatography-tandem mass spectrometry


Publication Details
Author(s): Meltretter J, Wüst J, Pischetsrieder M
Publisher: American Chemical Society
Publication year: 2013
Volume: 61
Pages range: 6971-6981
ISSN: 0021-8561

Abstract

Nonenzymatic post-translational protein modifications (nePTMs) result in changes of the protein structure that may severely influence physiological and technological protein functions. In the present study, ultrahigh-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) was applied for the systematic identification and site-specific analysis of nePTMs of β-lactoglobulin in processed milk. For this purpose, β-lactoglobulin, which had been heated with lactose under conditions to force nePTM formation (7 d/60 C), was screened for predicted modifications by using full scans and enhanced resolution scan experiments combined with enhanced product ion scans. Thus, the main glycation, glycoxidation, oxidation, and deamidation products of lysine, arginine, methionine, cysteine, tryptophan, and asparagine, as well as the N-terminus, were identified. Using these MS data, a very sensitive scheduled multiple reaction monitoring method suitable for the analysis of milk products was developed. Consequently, 14 different PTM structures on 25 binding sites of β-lactoglobulin were detected in different milk products. © 2013 American Chemical Society.



How to cite
APA: Meltretter, J., Wüst, J., & Pischetsrieder, M. (2013). Comprehensive analysis of nonenzymatic post-translational beta-lactoglobulin modifications in processed milk by ultrahigh-performance liquid chromatography-tandem mass spectrometry. Journal of Agricultural and Food Chemistry, 61, 6971-6981. https://dx.doi.org/10.1021/jf401549j

MLA: Meltretter, Jasmin, Johannes Wüst, and Monika Pischetsrieder. "Comprehensive analysis of nonenzymatic post-translational beta-lactoglobulin modifications in processed milk by ultrahigh-performance liquid chromatography-tandem mass spectrometry." Journal of Agricultural and Food Chemistry 61 (2013): 6971-6981.

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