SPIONs functionalized with small peptides for binding of lipopolysaccharide, a pathophysiologically relevant microbial product.

Journal article


Publication Details

Author(s): Karawacka W, Janko C, Unterweger H, Mühlberger M, Lyer S, Taccardi N, Mokhir A, Jira W, Peukert W, Boccaccini AR, Kolot M, Strauss R, Bogdan C, Alexiou C, Tietze R
Journal: Colloids and Surfaces B-Biointerfaces
Publication year: 2018
Volume: 174
Pages range: 95-102
ISSN: 0927-7765


Abstract

Systemic inflammation such as sepsis represents an acute life-threatening condition, to which often no timely remedy can be found. A promising strategy may be to functionalize magnetic nanoparticles with specific peptides, derived from the binding motives of agglutinating salivary proteins, that allow immobilization of pathogens. In this work, superparamagnetic iron oxide nanoparticles with stable polycondensed aminoalkylsilane layer were developed, to which the heterobifunctional linkers N-succinimidyl 3-(2-pyridyldithio)-propanoate (SDPD) and N-succinimidyl bromoacetate (SBA) were bound. These linkers were further chemoselectively reacted with the thiol group of singularly present cysteines of selected peptides. The resulting functional nanoparticles underwent a detailed physicochemical characterization. The biocompatibility of the primarily coated aminoalkylsilane particles was also investigated. To test the pathogen-binding efficacy of the particles, the lipopolysaccharide-immobilization capacity of the peptide-coated particles was compared with free peptides. Here, one particle-bound peptide species succeeded in capturing 90% of the toxin, whereas the degree of immobilization of the toxin with a system that varied in the sequence of the peptide dropped to 35%. With these promising results, we hope to develop extracorporeal magnetic clearance systems for removing pathogens from the human body in order to accelerate diagnosis and alleviate acute disease conditions such as sepsis.


FAU Authors / FAU Editors

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Boccaccini, Aldo R. Prof. Dr.-Ing.
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Bogdan, Christian Prof. Dr.
Lehrstuhl für Mikrobiologie und Infektionsimmunologie
Janko, Christina Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Lyer, Stefan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Mokhir, Andriy Prof. Dr.
Professur für Organische Chemie
Mühlberger, Marina
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Peukert, Wolfgang Prof. Dr.-Ing.
Lehrstuhl für Feststoff- und Grenzflächenverfahrenstechnik
Taccardi, Nicola Dr.
Lehrstuhl für Chemische Reaktionstechnik
Tietze, Rainer Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Unterweger, Harald Dr.-Ing.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie


External institutions
Max Rubner-Institut - Bundesforschungsinstitut für Ernährung und Lebensmittel
Tel Aviv University


How to cite

APA:
Karawacka, W., Janko, C., Unterweger, H., Mühlberger, M., Lyer, S., Taccardi, N.,... Tietze, R. (2018). SPIONs functionalized with small peptides for binding of lipopolysaccharide, a pathophysiologically relevant microbial product. Colloids and Surfaces B-Biointerfaces, 174, 95-102. https://dx.doi.org/10.1016/j.colsurfb.2018.11.002

MLA:
Karawacka, Weronika, et al. "SPIONs functionalized with small peptides for binding of lipopolysaccharide, a pathophysiologically relevant microbial product." Colloids and Surfaces B-Biointerfaces 174 (2018): 95-102.

BibTeX: 

Last updated on 2019-01-02 at 12:23