Polycatecholamine nanocoatings on stainless steel: the effect on attachment of human fibroblasts and platelets
Trzaskowska P, Rybak E, Trzaskowski M, Kopeć K, Krzemiński J, Podgórski R, Genc H, Civelek M, Cicha I (2026)
Publication Type: Journal article
Publication year: 2026
Journal
Book Volume: 17
Pages Range: 365-380
DOI: 10.3762/bjnano.17.25
Abstract
Polydopamine (PDA) is widely used to functionalize materials and enhance cell attachment. At the same time, the potential of the dopamine precursor tyrosine in its polymerized form (polytyrosine, PTYR) remains underexplored despite its biological activity. In this study, we developed nanostructured PDA and PTYR layers on stainless steel 316L via a novel in situ oxidation process and evaluated their physicochemical properties and cellular interactions at the nano/microscale. Surface characterization revealed that the polymeric coatings formed a homogenous layer with distinct topographical features and thickness in the nanometer range for PTYR and in the micrometer range in case of PDA. Compared to PDA, PTYR coatings exhibited a nanoparticulate surface morphology and higher stability under physiological conditions. Wettability, roughness, and amine group density were systematically analyzed to determine their influence on interactions with fibroblasts and platelets. Our results show that PTYR nanocoatings significantly reduced platelet adhesion and activation, whereas PDA coatings, due to their higher primary amine content, could in some cases enhance platelet adhesion. Furthermore, fibroblast attachment was primarily influenced by coating roughness, with a specific threshold beyond which adhesion did not increase or was negatively impacted. These findings highlight the potential of engineered PTYR nanocoatings for developing advanced hemocompatible surfaces for biomedical implants.
Authors with CRIS profile
Hatice Genc
Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik)
Mehtap Civelek
Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik)
Iwona Cicha
Professur für Nanomedizin
Involved external institutions
Poland (PL)How to cite
APA:
Trzaskowska, P., Rybak, E., Trzaskowski, M., Kopeć, K., Krzemiński, J., Podgórski, R.,... Cicha, I. (2026). Polycatecholamine nanocoatings on stainless steel: the effect on attachment of human fibroblasts and platelets. Beilstein Journal of Nanotechnology, 17, 365-380. https://doi.org/10.3762/bjnano.17.25
MLA:
Trzaskowska, Paulina, et al. "Polycatecholamine nanocoatings on stainless steel: the effect on attachment of human fibroblasts and platelets." Beilstein Journal of Nanotechnology 17 (2026): 365-380.
BibTeX: Download