Gal-2 Increases H3K4me3 and H3K9ac in Trophoblasts and Preeclampsia

Hahn L, Meister S, Mannewitz M, Beyer S, Corradini S, Hasbargen U, Mahner S, Jeschke U, Kolben T, Burges A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Biomolecules MDPI

Book Volume: 12

Article Number: 707

Journal Issue: 5

DOI: 10.3390/biom12050707

Abstract

Preeclampsia (PE) is a severe pregnancy disorder with a pathophysiology not yet com-pletely understood and without curative therapy. The histone modifications H3K4me3 and H3K9ac, as well as galectin-2 (Gal-2), are known to be decreased in PE. To gain a better understanding of the development of PE, the influence of Gal-2 on histone modification in trophoblasts and in syncytiali-sation was investigated. Immunohistochemical stains of 13 PE and 13 control placentas were corre-lated, followed by cell culture experiments. An analysis of H3K4me3 and H3K9ac was conducted, as well as cell fusion staining with E-cadherin and β-catenin—both after incubation with Gal-2. The expression of H3K4me3 and H3K9ac correlated significantly with the expression of Gal-2. Further-more, we detected an increase in H3K4me3 and H3K9ac after the addition of Gal-2 to BeWo/HVT cells. Moreover, there was increased fusion of HVT cells after incubation with Gal-2. Gal-2 is associated with the histone modifications H3K4me3 and H3K9ac in trophoblasts. Furthermore, syncyti-alisation increased after incubation with Gal-2. Therefore, we postulate that Gal-2 stimulates syn-cytialisation, possibly mediated by H3K4me3 and H3K9ac. Since Gal-2, as well as H3K4me3 and H3K9ac, are decreased in PE, the induction of Gal-2 might be a promising therapeutic target.

Involved external institutions

Klinikum der Universität München (LMU Klinikum) DE Germany (DE)

How to cite

APA:

Hahn, L., Meister, S., Mannewitz, M., Beyer, S., Corradini, S., Hasbargen, U.,... Burges, A. (2022). Gal-2 Increases H3K4me3 and H3K9ac in Trophoblasts and Preeclampsia. Biomolecules, 12(5). https://doi.org/10.3390/biom12050707

MLA:

Hahn, Laura, et al. "Gal-2 Increases H3K4me3 and H3K9ac in Trophoblasts and Preeclampsia." Biomolecules 12.5 (2022).

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