Three-dimensional vascularization of electrospun PCL/collagen-blend nanofibrous scaffolds in vivo

Klumpp D, Rudisile M, Kühnle RI, Heß A, Bitto FF, Arkudas A, Bleiziffer O, Boos A, Kneser U, Horch RE, Beier J (2012)

Publication Language: English

Publication Type: Journal article

Publication year: 2012

Journal

Journal of Biomedical Materials Research Part A Wiley

Book Volume: 100A

Pages Range: 2302-2311

Journal Issue: 9

DOI: 10.1002/jbm.a.34172

Abstract

Nanofiber scaffolds have proven their various advantages for tissue engineering and have been analyzed extensively. However, to date the three-dimensional pattern of vascularization inside nanofibrous scaffolds is unknown. This study introduces a novel method to visualize and quantify vascularization of electrospun nanofibrous PCL/collagen scaffolds in 3D in vivo. Randomly spun PCL/collagen blend and parallel aligned PCL/collagen blend/PEO scaffolds were analyzed for numbers and patterns of sprouting vessels inside the constructs using microCT scans at different time points. The image data derived from the microCT scans was converted into three-dimensional vessel trees. The aligned scaffold showed a significantly smaller number of sprouting vessels but vascularization in the center of the constructs occurred considerably earlier than in the nonwoven scaffold. Thus, for the first time the actual pattern of vascularization in nanofibrous scaffolds can be visualized three-dimensionally. These results demonstrate that the 3D pattern of vessel trees could be an essential parameter to evaluate nanofiber scaffolds for their suitability for tissue engineering as well as in vivo applications in general. Copyright © 2012 Wiley Periodicals, Inc.

Authors with CRIS profile

Dorothe Klumpp Professur für Plastische Chirurgie und Handchirurgie Andreas Heß Lehrstuhl für Pharmakologie und Toxikologie Franz-Ferdinand Bitto Professur für Plastische Chirurgie und Handchirurgie Andreas Arkudas Department of Plastic and Hand Surgery Anja Boos Department of Plastic and Hand Surgery Raymund Horch Professur für Plastische Chirurgie und Handchirurgie

Involved external institutions

Philipps-Universität Marburg DE Germany (DE)

How to cite

APA:

Klumpp, D., Rudisile, M., Kühnle, R.I., Heß, A., Bitto, F.-F., Arkudas, A.,... Beier, J. (2012). Three-dimensional vascularization of electrospun PCL/collagen-blend nanofibrous scaffolds in vivo. Journal of Biomedical Materials Research Part A, 100A(9), 2302-2311. https://doi.org/10.1002/jbm.a.34172

MLA:

Klumpp, Dorothe, et al. "Three-dimensional vascularization of electrospun PCL/collagen-blend nanofibrous scaffolds in vivo." Journal of Biomedical Materials Research Part A 100A.9 (2012): 2302-2311.

BibTeX: Download