Gensberger-Reigl S, Zenker H (2025)
Publication Type: Journal article
Publication year: 2025
Book Volume: 465
Article Number: 142034
DOI: 10.1016/j.foodchem.2024.142034
This study demonstrates the development and application of an ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC−HRMS) method for the rapid and sensitive identification of intact bovine milk proteins following simulated gastrointestinal infant digestion. The new method enables the differentiation between partially hydrolysed/modified and fully intact proteins. In the raw milk, intact α-lactalbumin was visible on SDS − PAGE until the end of the gastrointestinal digestion, while it was not detected with UHPLC−HRMS. Analysis of both raw and heated milk samples revealed that the method is applicable to various milk types. Interestingly, heated milk showed additional signals in the mass spectrum, indicating non-enzymatic post-translational modifications. The relative abundance of these proteoforms could be followed along digestion. These findings highlight the versatility and sensitivity of UHPLC−HRMS in elucidating protein structures and modifications, providing valuable insights into how simulated digestion affects milk protein composition.
APA:
Gensberger-Reigl, S., & Zenker, H. (2025). Detection of intact bovine milk proteins after simulated gastrointestinal infant digestion using UHPLC − HRMS. Food Chemistry, 465. https://doi.org/10.1016/j.foodchem.2024.142034
MLA:
Gensberger-Reigl, Sabrina, and Hannah Zenker. "Detection of intact bovine milk proteins after simulated gastrointestinal infant digestion using UHPLC − HRMS." Food Chemistry 465 (2025).
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