Genipin-Cross-Linked Silk Fibroin/Alginate Dialdehyde Hydrogel with Tunable Gelation Kinetics, Degradability, and Mechanical Properties: A Potential Candidate for Tissue Regeneration

Vaziri A, Vasheghani-Farahani E, Hosseinzadeh S, Bagheri F, Büchner M, Schubert DW, Boccaccini AR (2024)

Publication Language: English

Publication Type: Journal article

Publication year: 2024

Journal

Book Volume: 25

Pages Range: 2323-2337

Journal Issue: 4

DOI: 10.1021/acs.biomac.3c01203

Abstract

Genipin-cross-linked silk fibroin (SF) hydrogel is considered to be biocompatible and mechanically robust. However, its use remains a challenge for in situ forming applications due to its prolonged gelation process. In our attempt to facilitate the in situ fabrication of a genipin-mediated SF hydrogel, alginate dialdehyde (ADA) was utilized as a reinforcement template. Here, SF/ADA-based hydrogels with different compositions were synthesized covalently and ionically. Incorporating ADA into the SF hydrogel increased pore size (44.66-174.66 μm), porosity (61.59-80.40%), and the equilibrium swelling degree (7.60-30.17). Moreover, a wide range of storage modulus and compressive modulus were obtained by adjusting the proportions of SF and ADA networks within the hydrogel. The in vitro cell analysis using preosteoblast cells (MC3T3-E1) demonstrated the cytocompatibility of all hydrogels. Overall, the covalently and ionically cross-linked SF/ADA hydrogel represents a promising solution for in situ forming hydrogels for applications in tissue regeneration.

Authors with CRIS profile

Additional Organisation(s)

Involved external institutions

Tarbiat Modares University Iran, Islamic Republic of (IR) Shahid Beheshti University of Medical Sciences Iran, Islamic Republic of (IR)

How to cite

APA:

Vaziri, A., Vasheghani-Farahani, E., Hosseinzadeh, S., Bagheri, F., Büchner, M., Schubert, D.W., & Boccaccini, A.R. (2024). Genipin-Cross-Linked Silk Fibroin/Alginate Dialdehyde Hydrogel with Tunable Gelation Kinetics, Degradability, and Mechanical Properties: A Potential Candidate for Tissue Regeneration. Biomacromolecules, 25(4), 2323-2337. https://doi.org/10.1021/acs.biomac.3c01203

MLA:

Vaziri, Asmasadat, et al. "Genipin-Cross-Linked Silk Fibroin/Alginate Dialdehyde Hydrogel with Tunable Gelation Kinetics, Degradability, and Mechanical Properties: A Potential Candidate for Tissue Regeneration." Biomacromolecules 25.4 (2024): 2323-2337.

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