Amon L, Seichter A, Vurnek D, Heger L, Lächele L, Tochoedo NR, Kaszubowski T, Hatscher L, Baranska A, Tchitashvili G, Nimmerjahn F, Lehmann C, Dudziak D (2024)
Publication Type: Journal article
Publication year: 2024
Book Volume: 43
Article Number: 113949
Journal Issue: 3
DOI: 10.1016/j.celrep.2024.113949
Over the last decade, multiple studies have investigated the heterogeneity of murine conventional dendritic cells type 2 (cDC2s). However, their phenotypic similarity with monocytes and macrophages renders their clear identification challenging. By creating a protein atlas utilizing multiparameter flow cytometry, we show that ESAM+ cDC2s are a specialized feature of the spleen strongly differing in their proteome from other cDC2s. In contrast, all other tissues are populated by Clec12A+ cDC2s or Clec12A− cDC2s (high or low for Fcγ receptors, C-type lectin receptors, and CD11b, respectively), rendering Clec12A+ cDC2s classical sentinels. Further, expression analysis of CD301b, Clec12A, and FcγRIIB/III provides a conserved definition of cDC2 heterogeneity, including the discovery of putative FcγRIIB/III+ DC3s across tissues. Finally, our data reveal that cell identity (ontogeny) dictates the proteome that is further fine-tuned by the tissue environment on macrophages and dendritic cells (DCs), while monocytes and plasmacytoid DCs (pDCs) display subset intrinsic default settings.
APA:
Amon, L., Seichter, A., Vurnek, D., Heger, L., Lächele, L., Tochoedo, N.R.,... Dudziak, D. (2024). Clec12A, CD301b, and FcγRIIB/III define the heterogeneity of murine DC2s and DC3s. Cell Reports, 43(3). https://doi.org/10.1016/j.celrep.2024.113949
MLA:
Amon, Lukas, et al. "Clec12A, CD301b, and FcγRIIB/III define the heterogeneity of murine DC2s and DC3s." Cell Reports 43.3 (2024).
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