Müller-Seubert W, Ostermaier P, Horch RE, Distel L, Frey B, Erber R, Arkudas A (2023)
Publication Type: Journal article
Publication year: 2023
Book Volume: 13
Article Number: 1514
Journal Issue: 10
DOI: 10.3390/jpm13101514
Background: Irradiation plays an important role in the oncological treatment of various tumor entities. The aim of the study was to investigate the influence of different irradiation regimens on random-pattern flaps at the molecular and histopathological levels. Methods: Twenty-five rats underwent harvesting of bilateral random-pattern fasciocutaneous flaps. The right flaps received irradiation, while the left flaps served as non-irradiated intraindividual controls. Five rats served as a non-irradiated control group. Four different irradiation regimens with give rats each were tested: 20 Gy postoperatively, 3 × 12 Gy postoperatively, 20 Gy preoperatively, and 3 × 12 Gy preoperatively. Two weeks after surgery, HE staining and immunohistochemical staining for CD68 and ERG, as well as PCR analysis to detect Interleukin 6, HIF-1α, and VEGF, were performed. Results: A postoperative cumulative higher dose of irradiation appeared to result in an increase in necrosis, especially in the superficial layers of the flap compared to preoperative or single-stage irradiation. In addition, we observed increased expression of VEGF and HIF-1α in all irradiation groups. Conclusion: Even though no statistically significant differences were found between the different groups, there was a tendency for fractional postoperative irradiation with a higher total dose to have a more harmful effect compared to preoperative or single-dose irradiation.
APA:
Müller-Seubert, W., Ostermaier, P., Horch, R.E., Distel, L., Frey, B., Erber, R., & Arkudas, A. (2023). The Influence of Different Irradiation Regimens on Inflammation and Vascularization in a Random-Pattern Flap Model. Journal of Personalized Medicine, 13(10). https://doi.org/10.3390/jpm13101514
MLA:
Müller-Seubert, Wibke, et al. "The Influence of Different Irradiation Regimens on Inflammation and Vascularization in a Random-Pattern Flap Model." Journal of Personalized Medicine 13.10 (2023).
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