Anillin forms linear structures and facilitates furrow ingression after septin and formin depletion

Lebedev M, Chan FY, Lochner A, Bellessem J, Osório DS, Rackles E, Mikeladze-Dvali T, Carvalho AX, Zanin E (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Cell Reports Elsevier (Cell Press)

Book Volume: 42

Article Number: 113076

Journal Issue: 9

DOI: 10.1016/j.celrep.2023.113076

Abstract

During cytokinesis, a contractile ring consisting of unbranched filamentous actin (F-actin) and myosin II constricts at the cell equator. Unbranched F-actin is generated by formin, and without formin no cleavage furrow forms. In Caenorhabditis elegans, depletion of septin restores furrow ingression in formin mutants. How the cleavage furrow ingresses without a detectable unbranched F-actin ring is unknown. We report that, in this setting, anillin (ANI-1) forms a meshwork of circumferentially aligned linear structures decorated by non-muscle myosin II (NMY-2). Analysis of ANI-1 deletion mutants reveals that its disordered N-terminal half is required for linear structure formation and sufficient for furrow ingression. NMY-2 promotes the circumferential alignment of the linear ANI-1 structures and interacts with various lipids, suggesting that NMY-2 links the ANI-1 network with the plasma membrane. Collectively, our data reveal a compensatory mechanism, mediated by ANI-1 linear structures and membrane-bound NMY-2, that promotes furrowing when unbranched F-actin polymerization is compromised.

Authors with CRIS profile

Mikhail Lebedev Professur für Experimentelle Molekulare Zelldynamik Esther Zanin Professur für Experimentelle Molekulare Zelldynamik

Involved external institutions

Universidade do Porto PT Portugal (PT)

How to cite

APA:

Lebedev, M., Chan, F.-Y., Lochner, A., Bellessem, J., Osório, D.S., Rackles, E.,... Zanin, E. (2023). Anillin forms linear structures and facilitates furrow ingression after septin and formin depletion. Cell Reports, 42(9). https://dx.doi.org/10.1016/j.celrep.2023.113076

MLA:

Lebedev, Mikhail, et al. "Anillin forms linear structures and facilitates furrow ingression after septin and formin depletion." Cell Reports 42.9 (2023).

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