Transrectal Absorber Guide Raster-Scanning Optoacoustic Mesoscopy for Label-Free In Vivo Assessment of Colitis

Bühler A, Brown E, Paulus LP, Eckstein M, Thoma OM, Oraiopoulou ME, Rother U, Hörning A, Hartmann A, Neurath M, Wölfle J, Friedrich O, Waldner M, Knieling F, Bohndiek SEE, Regensburger A (2023)


Publication Type: Journal article, Original article

Publication year: 2023

Journal

DOI: 10.1002/advs.202300564

Abstract

Optoacoustic imaging (OAI) enables microscale imaging of endogenous chromophores such as hemoglobin at significantly higher penetration depths compared to other optical imaging technologies. Raster-scanning optoacoustic mesoscopy (RSOM) has recently been shown to identify superficial microvascular changes associated with human skin pathologies. In animal models, the imaging depth afforded by RSOM can enable entirely new capabilities for noninvasive imaging of vascular structures in the gastrointestinal tract, but exact localization of intra-abdominal organs is still elusive. Herein the development and application of a novel transrectal absorber guide for RSOM (TAG-RSOM) is presented to enable accurate transabdominal localization and assessment of colonic vascular networks in vivo. The potential of TAG-RSOM is demonstrated through application during mild and severe acute colitis in mice. TAG-RSOM enables visualization of transmural vascular networks, with changes in colon wall thickness, blood volume, and OAI signal intensities corresponding to colitis-associated inflammatory changes. These findings suggest TAG-RSOM can provide a novel monitoring tool in preclinical IBD models, refining animal procedures and underlines the capabilities of such technologies to address inflammatory bowel diseases in humans.

Authors with CRIS profile

Additional Organisation(s)

Related research project(s)

Involved external institutions

How to cite

APA:

Bühler, A., Brown, E., Paulus, L.-P., Eckstein, M., Thoma, O.-M., Oraiopoulou, M.-E.,... Regensburger, A. (2023). Transrectal Absorber Guide Raster-Scanning Optoacoustic Mesoscopy for Label-Free In Vivo Assessment of Colitis. Advanced Science. https://doi.org/10.1002/advs.202300564

MLA:

Bühler, Adrian, et al. "Transrectal Absorber Guide Raster-Scanning Optoacoustic Mesoscopy for Label-Free In Vivo Assessment of Colitis." Advanced Science (2023).

BibTeX: Download