Raman and infrared spectroscopy differentiate senescent from proliferating cells in a human dermal fibroblast 3D skin model
Eberhardt K, Matthaeus C, Winter D, Wiegand C, Hipler UC, Diekmann S, Popp J (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 142
Pages Range: 4405-4414
Journal Issue: 23
DOI: 10.1039/c7an00592j
Abstract
Senescent cells contribute to tissue aging and dysfunction. Therefore, detecting senescent cells in skin is of interest for skin tumor diagnostics and therapy. Here, we studied the transition into senescence of human dermal fibroblasts (HDFs) in a three-dimensional (3D) human fibroblast-derived matrix (FDM). Senescent and proliferating cells were imaged by Raman spectroscopy (RS) and Fourier transform infrared (FTIR) spectroscopy. The obtained averaged spectra were analyzed using PLS-LDA. For these 3D cultured cells, RS and FTIR could clearly distinguish senescent from proliferating cells. For both techniques, we detected senescence-associated alterations in almost all cellular macromolecules. Furthermore, we identified different biochemical properties of 3D compared to two-dimensional (2D) cultured cells, indicating that cells in their natural, skin-like 3D environment act differently than in (2D) cell cultivations in vitro. Compared to 2D cultured cells, cells grown in 3D models displayed a sharper contrast between the proliferating and senescent state, also affecting the abundance of biomolecules including nucleic acids. The training accuracies of both vibrational spectroscopic techniques were >96%, demonstrating the suitability of these label-free measurements for detecting these cellular states in 3D skin models.
Involved external institutions
Leibniz-Institut für Photonische Technologien e.V.
Germany (DE)
Universitätsklinikum Jena
Germany (DE)
Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut (FLI)
Germany (DE)
Germany (DE)
Universitätsklinikum Jena
Germany (DE)
Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut (FLI)
Germany (DE)
How to cite
APA:
Eberhardt, K., Matthaeus, C., Winter, D., Wiegand, C., Hipler, U.-C., Diekmann, S., & Popp, J. (2017). Raman and infrared spectroscopy differentiate senescent from proliferating cells in a human dermal fibroblast 3D skin model. Analyst, 142(23), 4405-4414. https://doi.org/10.1039/c7an00592j
MLA:
Eberhardt, Katharina, et al. "Raman and infrared spectroscopy differentiate senescent from proliferating cells in a human dermal fibroblast 3D skin model." Analyst 142.23 (2017): 4405-4414.
BibTeX: Download