Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids
Muehlig A, Jahn IJ, Heidler J, Jahn M, Weber K, Sheen P, Zimic M, Cialla-May D, Popp J (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 9
Article Number: 511
Journal Issue: 12
DOI: 10.3390/app9122511
Abstract
The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 μm for PZA and 21 μm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA.
Involved external institutions
Germany (DE)
Peru (PE)How to cite
APA:
Muehlig, A., Jahn, I.J., Heidler, J., Jahn, M., Weber, K., Sheen, P.,... Popp, J. (2019). Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids. Applied Sciences, 9(12). https://dx.doi.org/10.3390/app9122511
MLA:
Muehlig, Anna, et al. "Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids." Applied Sciences 9.12 (2019).
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