Multimodal nonlinear microscopy for therapy monitoring of cold atmospheric plasma treatment

Meyer T, Bae H, Hasse S, Winter J, Von Woedtke T, Schmitt M, Weltmann KD, Popp J (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Micromachines MDPI

Book Volume: 10

Article Number: 564

Journal Issue: 9

DOI: 10.3390/mi10090564

Abstract

Here we report on a non-linear spectroscopic method for visualization of cold atmospheric plasma (CAP)-induced changes in tissue for reaching a new quality level of CAP application in medicine via online monitoring of wound or cancer treatment. a combination of coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence lifetime imaging (2P-FLIM) and second harmonic generation (SHG) microscopy has been used for non-invasive and label-free detection of CAP-induced changes on human skin and mucosa samples. By correlation with histochemical staining, the observed local increase in fluorescence could be assigned to melanin. CARS and SHG prove the integrity of the tissue structure, visualize tissue morphology and composition. The influence of plasma effects by variation of plasma parameters e.g., duration of treatment, gas composition and plasma source has been evaluated. Overall quantitative spectroscopic markers could be identified for a direct monitoring of CAP-treated tissue areas, which is very important for translating CAPs into clinical routine.

Involved external institutions

Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE) Friedrich-Schiller-Universität Jena DE Germany (DE) Leibniz-Institut für Plasmaforschung und Technologie e.V. (INP Greifswald) / Leibniz Institute for Plasma Science and Technology DE Germany (DE)

How to cite

APA:

Meyer, T., Bae, H., Hasse, S., Winter, J., Von Woedtke, T., Schmitt, M.,... Popp, J. (2019). Multimodal nonlinear microscopy for therapy monitoring of cold atmospheric plasma treatment. Micromachines, 10(9). https://doi.org/10.3390/mi10090564

MLA:

Meyer, Tobias, et al. "Multimodal nonlinear microscopy for therapy monitoring of cold atmospheric plasma treatment." Micromachines 10.9 (2019).

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