Imaging flow cytometry with snapshot-mosaic cameras for characterization of Haematococcus pluvialis cells

Dittrich PG, Kraus D, Henkel T, Popp J, Rosenberger M, Notni G (2021)

Publication Type: Conference contribution

Publication year: 2021

Journal

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Publisher: SPIE

Book Volume: 11637

Conference Proceedings Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Event location: Virtual, Online, USA

ISBN: 9781510641099

DOI: 10.1117/12.2578559

Abstract

The extension of imaging flow cytometry (IFC) with snapshot-mosaic cameras is a novel approach for the in-flow characterization of bioparticles. With this innovative approach, cells at the single cell level as well as whole cell populations can be analyzed and counted. Spatial and spectrally resolving snapshot-mosaic cameras and real time capable multivariate data processing extend the possibilities for automated and in-flow characterization and analysis of mixed bioparticle populations. Cells move through a microfluidic chip for analysis and measurement as well as classification. For consistent imaging quality the cells passing an innovative 3-step microfluidic focusing unit. For the investigations we used the microalgae Haematococcus pluvialis (HP). These microalgae are used commercially to produce the red carotenoid pigment astaxanthin. Therefore, HP is suitable to practically demonstrate the usability of the developed system.

Involved external institutions

Technische Universität Ilmenau DE Germany (DE) Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE)

How to cite

APA:

Dittrich, P.-G., Kraus, D., Henkel, T., Popp, J., Rosenberger, M., & Notni, G. (2021). Imaging flow cytometry with snapshot-mosaic cameras for characterization of Haematococcus pluvialis cells. In Bonnie L. Gray, Holger Becker (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Virtual, Online, USA: SPIE.

MLA:

Dittrich, Paul-Gerald, et al. "Imaging flow cytometry with snapshot-mosaic cameras for characterization of Haematococcus pluvialis cells." Proceedings of the Microfluidics, BioMEMS, and Medical Microsystems XIX 2020, Virtual, Online, USA Ed. Bonnie L. Gray, Holger Becker, SPIE, 2021.

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