Tamoxifen-containing eye drops successfully trigger cre-mediated recombination in the entire eye
Schlecht A, Leimbeck SV, Tamm ER, Braunger BM (2016)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2016
Journal
Publisher: Springer New York LLC
Series: Advances in Experimental Medicine and Biology
Book Volume: 854
Pages Range: 495-500
DOI: 10.1007/978-3-319-17121-0_66
Abstract
Embryonic lethality in mice with targeted gene deletion is a major issue that can be circumvented by using Cre-loxP-based animal models. Various inducible Cre systems are available, e.g. such that are activated following tamoxifen treatment, and allow deletion of a specific target gene at any desired time point during the life span of the animal. In this study, we describe the efficiency of topical tamoxifen administration by eye drops using a Cre- reporter mouse strain (R26R). We report that tamoxifen-responsive CAGGCre-ER™ mice show a robust Cremediated recombination throughout the entire eye.
Involved external institutions
Germany (DE)How to cite
APA:
Schlecht, A., Leimbeck, S.V., Tamm, E.R., & Braunger, B.M. (2016). Tamoxifen-containing eye drops successfully trigger cre-mediated recombination in the entire eye. In (pp. 495-500). Springer New York LLC.
MLA:
Schlecht, Anja, et al. "Tamoxifen-containing eye drops successfully trigger cre-mediated recombination in the entire eye." Springer New York LLC, 2016. 495-500.
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