Light Intensity-Dependent Dysregulation of Retinal Reference Genes
Bielmeier CB, Schmitt SI, Braunger BM (2019)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2019
Journal
Publisher: Springer
Series: Advances in Experimental Medicine and Biology
Book Volume: 1185
Pages Range: 295-299
DOI: 10.1007/978-3-030-27378-1_48
Abstract
The degeneration of photoreceptors is a common hallmark of ocular diseases like retinitis pigmentosa (RP) or age-related macular degeneration (AMD). To experimentally induce photoreceptor degeneration, the light damage paradigm is frequently used. In this study we show that the exposure to high amounts of cool white light (10,000 lux, 1 h) resulted in a more than 11-fold higher apoptotic rate in the retina compared to light exposure with 5000 lux for 30 min. Consequently, exposure to intense light resulted in a significant downregulation of retinal mRNA expression levels of the reference genes Gapdh, Gnb2l, Rpl32, Rps9, Actb, Ubc or Tbp compared to untreated controls. Investigators performing light-induced photoreceptor degeneration should be aware of the fact that higher light intensities will result in a dysregulation of reference genes.
Involved external institutions
Germany (DE)How to cite
APA:
Bielmeier, C.B., Schmitt, S.I., & Braunger, B.M. (2019). Light Intensity-Dependent Dysregulation of Retinal Reference Genes. In (pp. 295-299). Springer.
MLA:
Bielmeier, Christina B., Sabrina I. Schmitt, and Barbara M. Braunger. "Light Intensity-Dependent Dysregulation of Retinal Reference Genes." Springer, 2019. 295-299.
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