Mining Naïve Rabbit Antibody Repertoires by Phage Display for Monoclonal Antibodies of Therapeutic Utility
Peng H, Nerreter T, Chang J, Qi J, Li X, Karunadharma P, Martinez GJ, Fallahi M, Soden J, Freeth J, Beerli RR, Grawunder U, Hudecek M, Rader C (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 429
Pages Range: 2954-2973
Journal Issue: 19
DOI: 10.1016/j.jmb.2017.08.003
Abstract
Owing to their high affinities and specificities, rabbit monoclonal antibodies (mAbs) have demonstrated value and potential primarily as basic research and diagnostic reagents, but, in some cases, also as therapeutics. To accelerate access to rabbit mAbs bypassing immunization, we generated a large naïve rabbit antibody repertoire represented by a phage display library encompassing > 10 billion independent antibodies in chimeric rabbit/human Fab format and validated it by next-generation sequencing. Panels of rabbit mAbs selected from this library against two emerging cancer targets, ROR1 and ROR2, revealed high diversity, affinity, and specificity. Moreover, ROR1- and ROR2-targeting rabbit mAbs demonstrated therapeutic utility as components of chimeric antigen receptor-engineered T cells, further corroborating the value of the naïve rabbit antibody library as a rich and virtually unlimited source of rabbit mAbs.
Involved external institutions
Scripps Research Institute (TSRI)
United States (USA) (US)
Julius-Maximilians-Universität Würzburg
Germany (DE)
Retrogenix
United Kingdom (GB)
NBE Therapeutics
Switzerland (CH)
United States (USA) (US)
Julius-Maximilians-Universität Würzburg
Germany (DE)
Retrogenix
United Kingdom (GB)
NBE Therapeutics
Switzerland (CH)
How to cite
APA:
Peng, H., Nerreter, T., Chang, J., Qi, J., Li, X., Karunadharma, P.,... Rader, C. (2017). Mining Naïve Rabbit Antibody Repertoires by Phage Display for Monoclonal Antibodies of Therapeutic Utility. Journal of Molecular Biology, 429(19), 2954-2973. https://dx.doi.org/10.1016/j.jmb.2017.08.003
MLA:
Peng, Haiyong, et al. "Mining Naïve Rabbit Antibody Repertoires by Phage Display for Monoclonal Antibodies of Therapeutic Utility." Journal of Molecular Biology 429.19 (2017): 2954-2973.
BibTeX: Download