Potential of Ni(II)-NTA-modified poly(ethylene imine) glycopolymers as carrier system for future dendritic cell-based immunotherapy

Hauptmann N, Pion M, Wehner R, Munoz-Fernandez MA, Schmitz M, Voit B, Appelhans D (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Biomacromolecules American Chemical Society

Book Volume: 15

Pages Range: 957-967

Journal Issue: 3

DOI: 10.1021/bm401845b

Abstract

Dendritic cells (DCs) play a crucial role in the development of cell-mediated immunotherapy due to their ability to induce and maintain strong immune responses. In our study, we evaluated a biocompatible Ni(II)-NTA-modified poly(ethylene imine) dendritic glycopolymer (Ni(II)-NTA-DG) as new carrier system to increase the antigen uptake into iDCs for future DC-based immunotherapy. Ni(II)-NTA-DG led to an increase in His6-Gp160 uptake in monocytes and iDCs, where His6-Gp160 is localized in the early endosomal and lysosomal compartments. Ni(II)-NTA-DG and the formed polyplexes induced an activation of iDCs, showing an increasing expression of costimulatory molecules CD86, CD80, and proinflammatory cytokines IL-6 and IL-8. Beside no influencing effect of Ni(II)-NTA-DG and polyplexes on the maturation of antigen-bearing DCs, the mature peptide bearing DCs remained their ability to migrate along a gradient of CCR7 ligands. Thus, Ni(II)-NTA-DG with advancing biological properties is a promising carrier system for the future application in DC-based immunotherapy. © 2014 American Chemical Society.

Involved external institutions

Leibniz-Institut für Polymerforschung Dresden e. V. DE Germany (DE) Hospital General Universitario Gregorio Marañón ES Spain (ES) Technische Universität Dresden DE Germany (DE)

How to cite

APA:

Hauptmann, N., Pion, M., Wehner, R., Munoz-Fernandez, M.-A., Schmitz, M., Voit, B., & Appelhans, D. (2014). Potential of Ni(II)-NTA-modified poly(ethylene imine) glycopolymers as carrier system for future dendritic cell-based immunotherapy. Biomacromolecules, 15(3), 957-967. https://dx.doi.org/10.1021/bm401845b

MLA:

Hauptmann, N., et al. "Potential of Ni(II)-NTA-modified poly(ethylene imine) glycopolymers as carrier system for future dendritic cell-based immunotherapy." Biomacromolecules 15.3 (2014): 957-967.

BibTeX: Download