Hybrid microscopy of human carotid atheroma by means of optical-resolution optoacoustic and non-linear optical microscopy
Seeger M, Karlas A, Soliman D, Pelisek J, Ntziachristos V (2017)
Publication Type: Conference contribution
Publication year: 2017
Journal
Publisher: SPIE
Book Volume: 10064
Conference Proceedings Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Event location: San Francisco, CA, USA
ISBN: 9781510605695
DOI: 10.1117/12.2254891
Abstract
Carotid atheromatosis is causally related to stroke, a leading cause of disability and death. We present the analysis of a human carotid atheroma using a novel hybrid microscopy system that combines optical-resolution optoacoustic (photoacoustic) microscopy and several non-linear optical microscopy modalities (second and third harmonic generation, as well as, two-photon excitation fluorescence) to achieve a multimodal examination of the extracted tissue within the same imaging framework. Our system enables the label-free investigation of atheromatous human carotid tissue with a resolution of about 1 μm and allows for the congruent interrogation of plaque morphology and clinically relevant constituents such as red blood cells, collagen, and elastin. Our data reveal mutual interactions between blood embeddings and connective tissue within the atheroma, offering comprehensive insights into its stage of evolution and severity, and potentially facilitating the further development of diagnostic tools, as well as treatment strategies.
Involved external institutions
Germany (DE)How to cite
APA:
Seeger, M., Karlas, A., Soliman, D., Pelisek, J., & Ntziachristos, V. (2017). Hybrid microscopy of human carotid atheroma by means of optical-resolution optoacoustic and non-linear optical microscopy. In Alexander A. Oraevsky, Lihong V. Wang (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. San Francisco, CA, USA: SPIE.
MLA:
Seeger, Markus, et al. "Hybrid microscopy of human carotid atheroma by means of optical-resolution optoacoustic and non-linear optical microscopy." Proceedings of the Photons Plus Ultrasound: Imaging and Sensing 2017, San Francisco, CA, USA Ed. Alexander A. Oraevsky, Lihong V. Wang, SPIE, 2017.
BibTeX: Download