Intravital hybrid optical-optoacoustic microscopy based on fiber-Bragg interferometry

Shnaiderman R, Wissmeyer G, Seeger M, Estrada H, Ntziachristos V (2018)

Publication Type: Conference contribution

Publication year: 2018

Journal

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Publisher: SPIE

Book Volume: 10494

Conference Proceedings Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Event location: San Francisco, CA, USA

ISBN: 9781510614734

DOI: 10.1117/12.2290281

Abstract

Optoacoustic microscopy (OAM) has enabled high-resolution, label-free imaging of tissues at depths not achievable with purely optical microscopy. However, widespread implementation of OAM into existing epi-illumination microscopy setups is often constrained by the performance and size of the commonly used piezoelectric ultrasound detectors. In this work, we introduce a novel acoustic detector based on a π-phase-shifted fiber Bragg grating (π-FBG) interferometer embedded inside an ellipsoidal acoustic cavity. The cavity enables seamless integration of epi-illumination OAM into existing microscopy setups by decoupling the acoustic and optical paths between the microscope objective and the sample. The cavity also acts as an acoustic condenser, boosting the sensitivity of the π-FBG and enabling cost effective CW-laser interrogation technique. We characterize the sensor's sensitivity and bandwidth and demonstrate hybrid OAM and second-harmonic imaging of phantoms and mouse tissue in vivo.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE)

How to cite

APA:

Shnaiderman, R., Wissmeyer, G., Seeger, M., Estrada, H., & Ntziachristos, V. (2018). Intravital hybrid optical-optoacoustic microscopy based on fiber-Bragg interferometry. In Lihong V. Wang, Alexander A. Oraevsky (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. San Francisco, CA, USA: SPIE.

MLA:

Shnaiderman, Rami, et al. "Intravital hybrid optical-optoacoustic microscopy based on fiber-Bragg interferometry." Proceedings of the Photons Plus Ultrasound: Imaging and Sensing 2018, San Francisco, CA, USA Ed. Lihong V. Wang, Alexander A. Oraevsky, SPIE, 2018.

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