Guidelines for DC preparation and flow cytometry analysis of mouse lymphohematopoietic tissues

Amon L, Dudziak D, Backer RAA, Clausen BE, Gmeiner C, Heger L, Jacobi L, Lehmann C, Probst HC, Seichter A, Tchitashvili G, Tochoedo NR, Trapaidze L, Vurnek D (2022)

Publication Type: Journal article

Publication year: 2022

Journal

European Journal of Immunology Wiley-VCH Verlag

DOI: 10.1002/eji.202249893

Abstract

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human DC from lymphoid organs, and various non-lymphoid tissues. Within this chapter, detailed protocols are presented that allow for the generation of single-cell suspensions from mouse lymphohematopoietic tissues including spleen, peripheral lymph nodes, and thymus, with a focus on the subsequent analysis of DC by flow cytometry. However, prepared single-cell suspensions can be subjected to other applications including sorting and cellular enrichment procedures, RNA sequencing, Western blotting, and many more. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.

Authors with CRIS profile

Lukas Amon Department of Dermatology Diana Dudziak Professur für die Biologie Dendritischer Zellen Lukas Heger Department of Dermatology Christian Lehmann Department of Dermatology Giorgi Tchitashvili Professur für die Biologie Dendritischer Zellen Nounagnon Romaric Tochoedo Department of Dermatology Lizi Trapaidze Department of Dermatology

Additional Organisation(s)

Interdisziplinäres Zentrum für Klinische Forschung (IZKF)

Related research project(s)

A087: Role of DC heterogeneity and natural antibodies for the disease outcome in cutaneous Leishmania infection Dec. 1, 2020 - Nov. 30, 2023 A087: Role of DC heterogeneity and natural antibodies for the disease outcome in cutaneous Leishmania infection July 1, 2020 - Nov. 30, 2023

Involved external institutions

Johannes Gutenberg-Universität Mainz (JGU) DE Germany (DE)

How to cite

APA:

Amon, L., Dudziak, D., Backer, R.A.A., Clausen, B.E., Gmeiner, C., Heger, L.,... Vurnek, D. (2022). Guidelines for DC preparation and flow cytometry analysis of mouse lymphohematopoietic tissues. European Journal of Immunology. https://doi.org/10.1002/eji.202249893

MLA:

Amon, Lukas, et al. "Guidelines for DC preparation and flow cytometry analysis of mouse lymphohematopoietic tissues." European Journal of Immunology (2022).

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