An In Vitro Approach for Investigating the Safety of Lipotransfer after Breast-Conserving Therapy

Promny T, Kutz CS, Jost T, Distel L, Kadam S, Schmid R, Arkudas A, Horch RE, Kengelbach-Weigand A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Original Authors: Theresa Promny, Chiara-Sophia Kutz, Tina Jost, Luitpold V. Distel, Sheetal Kadam, Rafael Schmid, Andreas Arkudas, Raymund E. Horch, Annika Kengelbach-Weigand

Book Volume: 12

Pages Range: 1284

Issue: 8

DOI: 10.3390/jpm12081284

Abstract

The application of lipotransfer after breast-conserving therapy (BCT) and irradiation in breast cancer patients is an already widespread procedure for reconstructing volume deficits of the diseased breast. Nevertheless, the safety of lipotransfer has still not been clarified yet due to contradictory data. The goal of this in vitro study was to further elucidate the potential effects of lipotransfer on the irradiated remaining breast tissue. The mammary epithelial cell line MCF-10A was co-cultured with the fibroblast cell line MRC-5 and irradiated with 2 and 5 Gy. Afterwards, cells were treated with conditioned medium (CM) from adipose-derived stem cells (ADSC), and the effects on the cellular functions of MCF-10A cells and on gene expression at the mRNA level in MCF-10A and MRC-5 cells were analyzed. Treatment with ADSC CM stimulated transmigration and invasion and decreased the surviving fraction of MCF-10A cells. Further, the expression of cytokines, extracellular, and mesenchymal markers was enhanced in mamary epithelial cells. Only an effect of ADSC CM on irradiated fibroblasts could be observed. The present data suggest epithelial–mesenchymal transition-like changes in the epithelial mammary breast cell line. Thus, the benefits of lipotransfer after BCT should be critically weighed against its possible risks for the affected patients.

Authors with CRIS profile

Theresa Promny Department of Plastic and Hand Surgery Chiara-Sophia Kutz Medizinische Fakultät Tina Jost Lehrstuhl für Strahlentherapie Luitpold Distel Department of Radiation Oncology Andreas Arkudas Professur für Plastische Chirurgie und Handchirurgie Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Annika Kengelbach-Weigand Department of Plastic and Hand Surgery

How to cite

APA:

Promny, T., Kutz, C.-S., Jost, T., Distel, L., Kadam, S., Schmid, R.,... Kengelbach-Weigand, A. (2022). An In Vitro Approach for Investigating the Safety of Lipotransfer after Breast-Conserving Therapy. Journal of Personalized Medicine, 12, 1284. https://doi.org/10.3390/jpm12081284

MLA:

Promny, Theresa, et al. "An In Vitro Approach for Investigating the Safety of Lipotransfer after Breast-Conserving Therapy." Journal of Personalized Medicine 12 (2022): 1284.

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