A 3D In Vivo Model for Studying Human Renal Cystic Tissue and Mouse Kidney Slices

Bichlmayer EM, Mahl L, Hesse L, Pion E, Haller V, Moehwald A, Hackl C, Werner JM, Schlitt HJ, Schwarz S, Kainz P, Brochhausen C, Groeger C, Steger F, Kölbl O, Daniel C, Amann KU, Kraus A, Buchholz B, Aung T, Härteis S (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Cells MDPI

Book Volume: 11

Journal Issue: 15

DOI: 10.3390/cells11152269

Abstract

(1) Background: Autosomal dominant polycystic kidney disease (ADPKD) is a frequent monogenic disorder that leads to progressive renal cyst growth and renal failure. Strategies to inhibit cyst growth in non-human cyst models have often failed in clinical trials. There is a significant need for models that enable studies of human cyst growth and drug trials. (2) Methods: Renal tissue from ADPKD patients who received a nephrectomy as well as adult mouse kidney slices were cultured on a chorioallantoic membrane (CAM) for one week. The cyst volume was monitored by microscopic and CT-based applications. The weight and angiogenesis were quantified. Morphometric and histological analyses were performed after the removal of the tissues from the CAM. (3) Results: The mouse and human renal tissue mostly remained vital for about one week on the CAM. The growth of cystic tissue was evaluated using microscopic and CT-based volume measurements, which correlated with weight and an increase in angiogenesis, and was accompanied by cyst cell proliferation. (4) Conclusions: The CAM model might bridge the gap between animal studies and clinical trials of human cyst growth, and provide a drug-testing platform for the inhibition of cyst enlargement. Real-time analyses of mouse kidney tissue may provide insights into renal physiology and reduce the need for animal experiments.

Authors with CRIS profile

Christoph Daniel Department of Nephropathology Kerstin Ute Amann Department of Nephropathology Andre Kraus Department of Medicine 4 – Nephrology and Hypertension Björn Buchholz Department of Medicine 4 – Nephrology and Hypertension Silke Härteis

Involved external institutions

Universität Regensburg DE Germany (DE) Universitätsklinikum Regensburg DE Germany (DE) KML Vision GmbH AT Austria (AT)

How to cite

APA:

Bichlmayer, E.M., Mahl, L., Hesse, L., Pion, E., Haller, V., Moehwald, A.,... Härteis, S. (2022). A 3D In Vivo Model for Studying Human Renal Cystic Tissue and Mouse Kidney Slices. Cells, 11(15). https://doi.org/10.3390/cells11152269

MLA:

Bichlmayer, Eva Marie, et al. "A 3D In Vivo Model for Studying Human Renal Cystic Tissue and Mouse Kidney Slices." Cells 11.15 (2022).

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