Immune Profiling of Polysaccharide Submicron Vesicles

Toma CC, Aloisi A, Bordoni V, Di Corato R, Rauner M, Cuniberti G, Delogu LG, Rinaldi R (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Biomacromolecules American Chemical Society

Book Volume: 19

Pages Range: 3560-3571

Journal Issue: 8

DOI: 10.1021/acs.biomac.8b00832

Abstract

Alginate (ALG) and chitosan (CS) have been extensively used for biomedical applications; however, data relative to immune responses exerted by them are scarce. We synthesized a submicron vesicle system (SV) displaying a CS shell over an ALG core. Intravenous injection of these promising carriers could be a possible route of delivery; therefore, we evaluated their impact on human peripheral blood mononuclear cells (PBMCs). By this ex vivo approach, we established how SV chemical-physical characteristics affected the immune cells in terms of cellular uptake, viability, and state of activation. By flow cytometry, we demonstrated that SVs were internalized by PBMCs with differential trends. No substantial necrotic and apoptotic signals were recorded, and SVs weakly affected activation status of PBMCs (concerning the markers CD69, CD25, CD80, and the cytokines TNF-α and IL-6), showing high immune biocompatibility and low immunomodulating properties. Our findings gain particular value toward the biomedical applications of SVs and make these polymer-based structures more attractive for translation into clinical uses.

Involved external institutions

Università del Salento IT Italy (IT) Consiglio Nazionale delle Ricerche (CNR) / National Research Council of Italy IT Italy (IT) Università degli Studi di Sassari (UNISS) IT Italy (IT) Technische Universität Dresden DE Germany (DE)

How to cite

APA:

Toma, C.C., Aloisi, A., Bordoni, V., Di Corato, R., Rauner, M., Cuniberti, G.,... Rinaldi, R. (2018). Immune Profiling of Polysaccharide Submicron Vesicles. Biomacromolecules, 19(8), 3560-3571. https://doi.org/10.1021/acs.biomac.8b00832

MLA:

Toma, Chiara C., et al. "Immune Profiling of Polysaccharide Submicron Vesicles." Biomacromolecules 19.8 (2018): 3560-3571.

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