Breaking Entry-and Species Barriers: LentiBOOST® Plus Polybrene Enhances Transduction Efficacy of Dendritic Cells and Monocytes by Adenovirus 5

Strack A, Deinzer A, Thirion C, Schrödel S, Dörrie J, Sauerer T, Steinkasserer A, Knippertz I (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Viruses MDPI

Book Volume: 14

Article Number: 92

Journal Issue: 1

DOI: 10.3390/v14010092

Abstract

Due to their ability to trigger strong immune responses, adenoviruses (HAdVs) in general and the serotype5 (HAdV-5) in particular are amongst the most popular viral vectors in research and clinical application. However, efficient transduction using HAdV-5 is predominantly achieved in coxsackie and adenovirus receptor (CAR)-positive cells. In the present study, we used the transduc-tion enhancer LentiBOOST® comprising the polycationic Polybrene to overcome these limitations. Using LentiBOOST® /Polybrene, we yielded transduction rates higher than 50% in murine bone marrow-derived dendritic cells (BMDCs), while maintaining their cytokine expression profile and their capability to induce T-cell proliferation. In human dendritic cells (DCs), we increased the transduction rate from 22% in immature (i)DCs or 43% in mature (m)DCs to more than 80%, without inducing cytotoxicity. While expression of specific maturation markers was slightly upregulated using LentiBOOST® /Polybrene on iDCs, no effect on mDC phenotype or function was observed. Moreover, we achieved efficient HAdV5 transduction also in human monocytes and were able to subsequently differentiate them into proper iDCs and functional mDCs. In summary, we introduce LentiBOOST® comprising Polybrene as a highly potent adenoviral transduction agent for new in-vitro applications in a set of different immune cells in both mice and humans.

Authors with CRIS profile

Astrid Strack Department of Immune Modulation Anja Deinzer Institute of General Practice Jan Dörrie Department of Dermatology Tatjana Sauerer Department of Dermatology Alexander Steinkasserer Professur für Experimentelle Dermatologie Ilka Knippertz Department of Immune Modulation

Involved external institutions

SIRION BIOTECH GmbH DE Germany (DE)

How to cite

APA:

Strack, A., Deinzer, A., Thirion, C., Schrödel, S., Dörrie, J., Sauerer, T.,... Knippertz, I. (2022). Breaking Entry-and Species Barriers: LentiBOOST® Plus Polybrene Enhances Transduction Efficacy of Dendritic Cells and Monocytes by Adenovirus 5. Viruses, 14(1). https://doi.org/10.3390/v14010092

MLA:

Strack, Astrid, et al. "Breaking Entry-and Species Barriers: LentiBOOST® Plus Polybrene Enhances Transduction Efficacy of Dendritic Cells and Monocytes by Adenovirus 5." Viruses 14.1 (2022).

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