Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats

Werry JE, Müller S, Wehrhan F, Geppert CI, Frohwitter G, Kaemmerer PW, Möst T, Lutz R, Homm A, Kesting MR, Weber M, Ries J (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Applied Sciences MDPI

Book Volume: 11

Journal Issue: 12

DOI: 10.3390/app11125589

Abstract

This study aimed to investigate accuracy in different sectional planes of the TMA Grand Master (3DHISTECH) Workstation in various soft tissue samples collected from Wistar rats. A total of 108 animals were sacrificed and 963 tissue specimens collected from 12 soft-tissue types. A total of 3307 tissue cores were punched and transferred into 40 recipient TMA blocks. Digital image analysis was performed. Core loss showed a significant correlation with tissue type and was highest in skin tissue (p < 0.001), renal medulla and femoral artery, nerve, and vein bundle (p < 0.01). Overall, 231 of 3307 tissue cores (7.0%) were lost. Hit rate analysis was performed in 1852 punches. The target was hit completely, partially and missed totally by 89.4%, 7.2% and 2.2%. A total of 54.5% of punches had good accuracy with less than 200 mu m deviation from the centre of the targeted region and 92.6% less than 500 mu m. Accuracy decreases with greater sectional depth. In the deepest sectional plane of roughly 0.5 mm median depth, almost 90% of cores had a deviation below 500 mu m. Recommendations for automated TMA creation are given in this article. The ngTMA(R)-method has proven accurate and reliable in different soft tissues, even in deeper sectional layers.

Authors with CRIS profile

Jan-Erik Werry Department of Oral and Cranio-Maxillofacial Surgery Stefan Müller Department of Oral and Cranio-Maxillofacial Surgery Falk Wehrhan Department of Oral and Cranio-Maxillofacial Surgery Carol-Immanuel Geppert Institute of Pathology Gesche Frohwitter Department of Oral and Cranio-Maxillofacial Surgery Tobias Möst Department of Oral and Cranio-Maxillofacial Surgery Rainer Lutz Department of Oral and Cranio-Maxillofacial Surgery Andi Homm Department of Oral and Cranio-Maxillofacial Surgery Marco Rainer Kesting Lehrstuhl für Zahnmedizin, insbesondere Mund-, Kiefer-, und Gesichtschirurgie Manuel Weber Department of Oral and Cranio-Maxillofacial Surgery Jutta Ries Department of Oral and Cranio-Maxillofacial Surgery

Involved external institutions

Johannes Gutenberg-Universität Mainz (JGU) DE Germany (DE)

How to cite

APA:

Werry, J.-E., Müller, S., Wehrhan, F., Geppert, C.-I., Frohwitter, G., Kaemmerer, P.W.,... Ries, J. (2021). Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats. Applied Sciences, 11(12). https://doi.org/10.3390/app11125589

MLA:

Werry, Jan-Erik, et al. "Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats." Applied Sciences 11.12 (2021).

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