An Inverse Thermogelling Bioink Based on an ABA-Type Poly(2-oxazoline) Amphiphile

Hahn L, Karakaya E, Zorn T, Sochor B, Maier M, Stahlhut P, Forster S, Fischer K, Seiffert S, Pöppler AC, Detsch R, Luxenhofer R (2021)

Publication Type: Journal article

Publication year: 2021


DOI: 10.1021/acs.biomac.1c00427


Hydrogels are key components in several biomedical research areas such as drug delivery, tissue engineering, and biofabrication. Here, a novel ABA-type triblock copolymer comprising poly(2-methyl-2-oxazoline) as the hydrophilic A blocks and poly(2-phenethyl-2-oxazoline) as the aromatic and hydrophobic B block is introduced. Above the critical micelle concentration, the polymer self-assembles into small spherical polymer micelles with a hydrodynamic radius of approx 8-8.5 nm. Interestingly, this specific combination of hydrophilic and hydrophobic aromatic moieties leads to rapid thermoresponsive inverse gelation at polymer concentrations above a critical gelation concentration (20 wt %) into a macroporous hydrogel of densely packed micelles. This hydrogel exhibited pronounced viscoelastic solid-like properties, as well as extensive shear-thinning, rapid structure recovery, and good strain resistance properties. Excellent 3D-printability of the hydrogel at lower temperature opens a wide range of different applications, for example, in the field of biofabrication. In preliminary bioprinting experiments using NIH 3T3 cells, excellent cell viabilities of more than 95% were achieved. The particularly interesting feature of this novel material is that it can be used as a printing support in hybrid bioink systems and sacrificial bioink due to rapid dissolution at physiological conditions.

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Hahn, L., Karakaya, E., Zorn, T., Sochor, B., Maier, M., Stahlhut, P.,... Luxenhofer, R. (2021). An Inverse Thermogelling Bioink Based on an ABA-Type Poly(2-oxazoline) Amphiphile. Biomacromolecules.


Hahn, Lukas, et al. "An Inverse Thermogelling Bioink Based on an ABA-Type Poly(2-oxazoline) Amphiphile." Biomacromolecules (2021).

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