A silicon nitride ISFET based immunosensor for tumor necrosis factor-alpha detection in saliva. A promising tool for heart failure monitoring

Ben Halima H, Bellagambi FG, Alcacer A, Pfeiffer N, Heuberger A, Hangouët M, Zine N, Bausells J, Elaissari A, Errachid A (2021)


Publication Type: Journal article

Publication year: 2021

Journal

Book Volume: 1161

Article Number: 338468

DOI: 10.1016/j.aca.2021.338468

Abstract

According to the European statistics, approximately 26 million patients worldwide suffer from heart failure (HF), and this number seems to be steadily increasing. Inflammation plays a central role in the development of HF, and the pro-inflammatory cytokine Tumor necrosis factor-α (TNF-α) represents inflammation gold-standard biomarker. Early detection plays a crucial role for the prognosis and treatment of HF. An Ion Sensitive Field Effect Transistor (ISFET) based on silicon nitride transducer and biofunctionalized with anti-TNF-α antibody for label-free detection of salivary TNF-α is proposed. Electrochemical impedance spectroscopy (EIS) was used for TNF-α detection. Our ImmunoFET offered a detection limit of 1 pg mL−1, with an analytical reproducibility expressed by a coefficient of variance (CV) resulted < 10% for the analysis of saliva samples, and an analyte recovery of 94 ± 6%. In addition, it demonstrated high selectivity when compared to other HF biomarkers such as Inteleukin-10, N-terminal pro B-type natriuretic peptide, and Cortisol. Finally, ImmunoFET accuracy in determining the unknown concentration of TNF-α was successfully tested in saliva samples by performing standard addition method. The proposed ImmunoFET showed great promise as a complementary tool for biomedical application for HF monitoring by a non-invasive, rapid and accurate assessment of TNF-α.

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APA:

Ben Halima, H., Bellagambi, F.G., Alcacer, A., Pfeiffer, N., Heuberger, A., Hangouët, M.,... Errachid, A. (2021). A silicon nitride ISFET based immunosensor for tumor necrosis factor-alpha detection in saliva. A promising tool for heart failure monitoring. Analytica Chimica Acta, 1161. https://doi.org/10.1016/j.aca.2021.338468

MLA:

Ben Halima, Hamdi, et al. "A silicon nitride ISFET based immunosensor for tumor necrosis factor-alpha detection in saliva. A promising tool for heart failure monitoring." Analytica Chimica Acta 1161 (2021).

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