B Cell Speed and B-FDC Contacts in Germinal Centers Determine Plasma Cell Output via Swiprosin-1/EFhd2

Reimer D, Meyer-Hermann M, Rakhymzhan A, Steinmetz T, Tripal P, Thomas J, Böttcher M, Mougiakakos D, Schulz S, Urbanczyk S, Hauser AE, Niesner RA, Mielenz D (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Cell Reports Elsevier (Cell Press)

Book Volume: 32

Article Number: 108030

Journal Issue: 6

DOI: 10.1016/j.celrep.2020.108030

Abstract

Plasma cells secreting affinity-matured antibodies develop in germinal centers (GCs), where B cells migrate persistently and directionally over defined periods of time. How modes of GC B cell migration influence plasma cell development remained unclear. Through genetic deletion of the F-actin bundling protein Swiprosin-1/EF-hand domain family member 2 (EFhd2) and by two-photon microscopy, we show that EFhd2 restrains B cell speed in GCs and hapten-specific plasma cell output. Modeling the GC reaction reveals that increasing GC B cell speed promotes plasma cell generation. Lack of EFhd2 also reduces contacts of GC B cells with follicular dendritic cells in vivo. Computational modeling uncovers that both GC output and antibody affinity depend quantitatively on contacts of GC B cells with follicular dendritic cells when B cells migrate more persistently. Collectively, our data explain how GC B cells integrate speed and persistence of cell migration with B cell receptor affinity.

Authors with CRIS profile

Dorothea Reimer Department of Molecular Immunology Tobit Steinmetz Professur für Immunologie Philipp Tripal Optical Imaging Center Erlangen (OICE) Martin Böttcher Department of Medicine 5 – Haematology and Oncology Dimitrios Mougiakakos Professur für Hämatologie/Onkologie mit dem Schwerpunkt Tumorimmunologie Sebastian Schulz Professur für Immunologie Sophia Urbanczyk Department of Molecular Immunology Dirk Mielenz Medizinische Fakultät

Involved external institutions

Helmholtz-Zentrum für Infektionsforschung (HZI) DE Germany (DE) Deutsches Rheuma-Forschungszentrum (DRFZ) DE Germany (DE)

How to cite

APA:

Reimer, D., Meyer-Hermann, M., Rakhymzhan, A., Steinmetz, T., Tripal, P., Thomas, J.,... Mielenz, D. (2020). B Cell Speed and B-FDC Contacts in Germinal Centers Determine Plasma Cell Output via Swiprosin-1/EFhd2. Cell Reports, 32(6). https://doi.org/10.1016/j.celrep.2020.108030

MLA:

Reimer, Dorothea, et al. "B Cell Speed and B-FDC Contacts in Germinal Centers Determine Plasma Cell Output via Swiprosin-1/EFhd2." Cell Reports 32.6 (2020).

BibTeX: Download