Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Cossarizza A, Acs A, Adam D, Adam-Klages S, Agace WW, Aghaeepour N, Akdis M, Allez M, Almeida LN, Alvisi G, Anderson G, Andrä I, Annunziato F, Anselmo A, Bacher P, Baldari CT, Bari S, Barnaba V, Barros-Martins J, Battistini L, Bauer W, Baumgarth N, Baumjohann D, Baying B, Bebawy M, Becher B, Beisker W, Benes V, Beyaert R, Blanco A, Boardman DA, Bogdan C, Borger JG, Borsellino G, Boulais PE, Bradford JA, Brenner D, Brinkman RR, Brooks AE, Busch DH, Büscher M, Bushnell TP, Calzetti F, Cameron G, Cammarata I, Cao X, Cardell SL, Casola S, Cassatella MA, Cavani A, Celada A, Chatenoud L, Chattopadhyay PK, Chow S, Christakou E, Čičin-Šain L, Clerici M, Colombo FS, Cook L, Cooke A, Cooper AM, Corbett AJ, Cosma A, Cosmi L, Coulie PG, Cumano A, Cvetkovic L, Dang-Heine C, Davey MS, Davies D, De Biasi S, Del Zotto G, Dela Cruz GV, Delacher M, Della Bella S, Dellabona P, Deniz G, Dessing M, Di Santo JP, Dieli F, Dolf A, Dress RJ, Dudziak D, Dustin M, Dutertre CA, Ebner F, Eckle SB, Edinger M, Eede P, Ehrhardt GR, Eich M, Engel P, Engelhardt B, Erdei A, Esser C, Everts B, Evrard M, Falk CS, Fehniger TA, Felipo-Benavent M, Ferry H, Feuerer M, Filby A, Filkor K, Fillatreau S, Follo M, Förster I, Foster J, Foulds GA, Frehse B, Frenette PS, Fritzsche W, Galbraith DW, Gangaev A, Garbi N, Gaudilliere B, Gazzinelli RT, Geginat J, Gerner W, Gherardin NA, Ghoreschi K, Gibellini L, Ginhoux F, Goda K, Godfrey DI, Goettlinger C, González-Navajas JM, Goodyear CS, Gori A, Grogan JL, Grummitt D, Haftmann C, Hahn J, Hammad H, Hämmerling G, Hansmann L, Hansson G, Harpur CM, Hartmann S, Hauser A, Haviland DL, Hedley D, Herrera G, Herrmann M, Hess C, Höfer T, Hoffmann P, Hogquist K, Holland T, Höllt T, Holmdahl R, Hombrink P, Houston JP, Hoyer BF, Huang B, Huang FP, Huber JE, Huehn J, Hundemer M, Hunter CA, Hwang WY, Iannone A, Ingelfinger F, Ivison SM, Jäck HM, Jávega B, Jonjic S, Kalina T, Kamradt T, Kaufmann SH, Keller B, Ketelaars SL, Khalilnezhad A, Khan S, Kisielow J, Klenerman P, Knopf J, Koay HF, Kobow K, Kolls JK, Kong WT, Kopf M, Korn T, Kriegsmann K, Kristyanto H, Kroneis T, Krueger A, Kühne J, Kukat C, Kunkel D, Kunze-Schumacher H, Kurosaki T, Kurts C, Kvistborg P, Kwok I, Landry J, Lantz O, Lanuti P, LaRosa F, Lehuen A, LeibundGut-Landmann S, Leipold MD, Leung LY, Levings MK, Liotta F, Litwin V, Liu Y, Ljunggren HG, Lohoff M, Lombardi G, Lopez L, López-Botet M, Lovett-Racke AE, Lubberts E, Luche H, Ludewig B, Lugli E, Lunemann S, Maecker HT, Maggi L, Maguire O, Mair F, Mair KH, Mantovani A, Manz RA, Marshall AJ, Martínez-Romero A, Martrus G, Marventano I, Maslinski W, Matarese G, Mattioli AV, Maueröder C, Mazzoni A, McCluskey J, McGuire HM, McInnes IB, Melzer S, Mielenz D, Miller SD, Mills KH, Minderman H, Mjösberg J, Moore J, Moran B, Moretta L, Mosmann TR, Müller S, Multhoff G, Munoz LE, Münz C, Nakayama T, Nasi M, Neumann K, Ng LG, Nourshargh S, Núñez G, O'Connor JE, Ochel A, Oja A, Ordonez D, Orfao A, Orlowski-Oliver E, Ouyang W, Oxenius A, Palankar R, Pattanapanyasat K, Paulsen M, Pavlinic D, Penter L, Peterson P, Peth C, Petriz J, Piancone F, Pickl WF, Piconese S, Pinti M, Pockley AG, Podolska M, Poon Z, Pracht K, Prinz I, Pucillo CE, Quataert SA, Quatrini L, Quinn KM, Radbruch H, Radstake TR, Rahmig S, Rahn HP, Rajwa B, Ravichandran G, Raz Y, Rebhahn JA, Recktenwald D, Reimer D, Reis e Sousa C, Remmerswaal EB, Richter L, Rico LG, Riddell A, Rieger AM, Robinson JP, Rubartelli A, Ruland J, Saalmüller A, Saeys Y, Saito T, Sakaguchi S, Sala-de-Oyanguren F, Samstag Y, Sanderson S, Sandrock I, Santoni A, Sanz RB, Saresella M, Sautes-Fridman C, Sawitzki B, Schadt L, Scheffold A, Scherer HU, Schiemann M, Schildberg FA, Schimisky E, Schlitzer A, Schlosser J, Schmid S, Schmitt S, Schober K, Schraivogel D, Schuh W, Schüler T, Schulte R, Schulz S, Scottá C, Scott-Algara D, Sester DP, Shankey TV, Silva-Santos B, Simon AK, Sitnik KM, Sozzani S, Speiser DE, Spidlen J, Stahlberg A, Stall AM, Stanley N, Stark R, Steinmetz T, Stockinger H, Takahama Y, Takeda K, Tan L, Tárnok A, Tiegs G, Toldi G, Traggiai E, Trebak M, Tree TI, Trotter J, Trowsdale J, Tsoumakidou M, Ulrich H, Urbanczyk S, van de Veen W, van den Broek M, van der Pol E, Van Gassen S, Van Isterdael G, van Lier RA, Veldhoen M, Vento-Asturias S, Vieira P, Vöhringer D, Volk HD, von Borstel A, von Volkmann K, Waisman A, Walker RV, Wallace PK, Wang SA, Wang XM, Ward MD, Ward-Hartstonge KA, Warnatz K, Warnes G, Warth S, Waskow C, Watson JV, Watzl C, Wegener L, Weisenburger T, Wienands J, Wilharm A, Wilkinson RJ, Willimsky G, Wing JB, Winkelmann R, Winkler T, Wirz OF, Wong A, Wurst P, Yang JH, Yang J, Yazdanbakhsh M, Yu L, Yue A, Zhang H, Zhao Y, Ziegler SM, Zielinski C, Zimmermann J, Zychlinsky A

Publication Type: Journal article

Journal

Book Volume: 49

Pages Range: 1457-1973

Journal Issue: 10

DOI: 10.1002/eji.201970107

Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

Authors with CRIS profile

Involved external institutions

Universität zu Lübeck Germany (DE) Medizinische Universität Wien Austria (AT) University of Zurich / Universität Zürich (UZH) Switzerland (CH) University of California Davis (UCDAVIS) United States (USA) (US) Deutsches Rheuma-Forschungszentrum (DRFZ) Germany (DE) Stanford University United States (USA) (US) Christian-Albrechts-Universität zu Kiel Germany (DE) National Cancer Centre Singapore (NCCS) Singapore (SG) University of Birmingham United Kingdom (GB) Università degli Studi di Modena e Reggio Emilia (UNIMORE) Italy (IT) Universitätsklinikum Schleswig-Holstein (UKSH) Germany (DE) Fondazione Santa Lucia IRCCS Italy (IT) University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot France (FR) Humanitas University Italy (IT) Università degli Studi di Firenze / University of Florence Italy (IT) University of Technology Sydney (UTS) Australia (AU) European Molecular Biology Laboratory (EMBL) Germany (DE) University of Gothenburg / Göteborgs universitet Sweden (SE) Technische Universität München (TUM) Germany (DE) Nankai University (NKU) / 南开大学 China (CN) Miltenyi Biotec GmbH Germany (DE) Humanitas Research Hospital / IRCCS Istituto Clinico Humanitas Italy (IT) Thermo Fisher Scientific Inc. United States (USA) (US) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich Germany (DE) University of Rochester Medical Center Rochester, NY United States (USA) (US) Princess Margaret Cancer Centre / Princess Margaret Hospital Canada (CA) Monash University Australia (AU) Leibniz-Gemeinschaft Germany (DE) Medizinische Hochschule Hannover (MHH) / Hannover Medical School Germany (DE) Luxembourg Institute of Health (CRP-Santé) Luxembourg (LU) Technical University of Denmark / Danmarks Tekniske Universitet (DTU) Denmark (DK) IFOM - FIRC Institute of Molecular Oncology Italy (IT) Charité - Universitätsmedizin Berlin Germany (DE) University of Oxford United Kingdom (GB) Université Catholique de Louvain (UCL) Belgium (BE) Institut Pasteur France (FR) Singapore Immunology Network (SIgN) Singapore (SG) The University of Melbourne Australia (AU) Università degli Studi di Palermo Italy (IT) Sony Europe Limited Germany (DE) San Rafaele Scientific Institute Italy (IT) Freie Universität Berlin Germany (DE) University of Leicester United Kingdom (GB) Istituto Giannina Gaslini Italy (IT) University of Cambridge United Kingdom (GB) Istanbul University / İstanbul Üniversitesi Turkey (TR) The Francis Crick Institute United Kingdom (GB) BC Children's Hospital Research Institute Canada (CA) Duke-NUS Medical School / 杜克—国大医学研究生院 Singapore (SG) Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH) Germany (DE) Universitat de Barcelona (UB) / University of Barcelona Spain (ES) University of Copenhagen Denmark (DK) RCI - Regensburger Centrum für Interventionelle Immunologie Germany (DE) Eötvös Loránd University / Eötvös Loránd Tudományegyetem (ELTE) Hungary (HU) Università degli studi di Milano Italy (IT) Rheinische Friedrich-Wilhelms-Universität Bonn Germany (DE) University of Verona / Università degli Studi di Verona Italy (IT) Leibniz-Institut für umweltmedizinische Forschung (IUF) / Leibniz Research Institute for Environmental Medicine Germany (DE) Ludwig-Maximilians-Universität (LMU) Germany (DE) Albert-Ludwigs-Universität Freiburg Germany (DE) Università degli Studi di Siena (UNISI) / University of Siena Italy (IT) RCCS Fondazione Don Carlo Gnocchi Onlus Italy (IT) University of Paris 5 - René Descartes / Université Paris V René Descartes France (FR) King’s College London United Kingdom (GB) University College Dublin (UCD) Ireland (IE) MDQuest Kft. Hungary (HU) Owl Biomedical United States (USA) (US) Albert Einstein College of Medicine United States (USA) (US) University of British Columbia Canada (CA) NYU Langone Medical Center United States (USA) (US) Università degli studi "La Sapienza" Italy (IT) NIHMP - National Institute for Health, Migration and Poverty Italy (IT) University of Auckland New Zealand (NZ) Universität zu Köln Germany (DE) Universiteit Gent (UGent) / Ghent University Belgium (BE) University of Toronto Canada (CA) Newcastle University United Kingdom (GB) Genentech Inc. United States (USA) (US) Leiden University Medical Center Netherlands (NL) Leibniz-Institut für Photonische Technologien e.V. Germany (DE) Universitätsklinikum Regensburg Germany (DE) Nottingham Trent University United Kingdom (GB) University of California Los Angeles (UCLA) United States (USA) (US) Karolinska University Hospital / Karolinska Universitetssjukhuset Sweden (SE) Prince Felipe Research Center / Centro de Investigación Príncipe Felipe (CIPF) Spain (ES) University of València / Universitat de València Spain (ES) Helmholtz-Zentrum für Infektionsforschung (HZI) Germany (DE) Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital (NKI / NKI-AVL) Netherlands (NL) The Methodist Hospital System United States (USA) (US) Oswaldo Cruz Foundation / Fundação Oswaldo Cruz (FIOCRUZ) Brazil (BR) Berliner Institut für Gesundheitsforschung (BIH) Germany (DE) Washington University in St. Louis United States (USA) (US) Deutsches Krebsforschungszentrum (DKFZ) Germany (DE) Murdoch Childrens Research Institute Australia (AU) University of Arizona United States (USA) (US) Universität Bern Switzerland (CH) University of Glasgow United Kingdom (GB) Institution Nazional Genetica Molecolare Ingm Italy (IT) Veterinärmedizinische Universität Wien (Vetmeduni Vienna) / University of Veterinary Medicine, Vienna Austria (AT) Hospital General Universitario de Alicante Spain (ES)

How to cite

APA:

Cossarizza, A., Acs, A., Adam, D., Adam-Klages, S., Agace, W.W., Aghaeepour, N.,... Zychlinsky, A. (2019). Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology, 49(10), 1457-1973. https://doi.org/10.1002/eji.201970107

MLA:

Cossarizza, Andrea, et al. "Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)." European Journal of Immunology 49.10 (2019): 1457-1973.

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