Muheim-Lenz R (2004)
Publication Status: Published
Publication Type: Journal article
Publication year: 2004
Publisher: OXFORD UNIV PRESS
Book Volume: 32
Pages Range: 6696-6705
Journal Issue: 22
DOI: 10.1093/nar/gkh990
We examined whether the human nucleotide excision repair complex, which is specialized on the removal of bulky DNA adducts, also displays a correcting activity on base mismatches. The cytosine/cytosine (C/C) lesion was used as a model substrate to monitor the correction of base mismatches in human cells. Fibroblasts with different repair capabilities were transfected with shuttle vectors that contain a site-directed C/C mismatch in the replication origin, accompanied by an additional C/C mismatch in one of the flanking sequences that are not essential for replication. Analysis of the vector progeny obtained from these doubly modified substrates revealed that C/C mismatches were eliminated before DNA synthesis not only in the repair-proficient background, but also when the target cells carried a genetic defect in long-patch mismatch repair, in nucleotide excision repair, or when both pathways were deleted. Furthermore, cells deficient for long-patch mismatch repair as well as a cell line that combines mismatch and nucleotide excision repair defects were able to correct multiple C/C mispairs, placed at distances of 21-44 nt, in an independent manner, such that the removal of each lesion led to individual repair patches. These results support the existence of a concurrent short-patch mechanism that rectifies C/C mismatches.
APA:
Muheim-Lenz, R. (2004). Short-patch correction of C/C mismatches in human cells. Nucleic Acids Research, 32(22), 6696-6705. https://dx.doi.org/10.1093/nar/gkh990
MLA:
Muheim-Lenz, Regula. "Short-patch correction of C/C mismatches in human cells." Nucleic Acids Research 32.22 (2004): 6696-6705.
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