Poly-3-hydroxyoctanoate P(3HO), a Medium Chain Length Polyhydroxyalkanoate Homopolymer from Pseudomonas mendocina

Rai R, Yunos DM, Boccaccini AR, Knowles JC, Barker IA, Howdle SM, Tredwell GD, Keshavarz T, Roy I (2011)

Publication Type: Journal article

Publication year: 2011

Journal

Biomacromolecules American Chemical Society

Publisher: American Chemical Society

Pages Range: 2126-2136

Journal Issue: 12

DOI: 10.1021/bm2001999

Abstract

Pseudomonas mendocina was found to produce a unique homopolymer of poly(3-hydroxyoctanoate), P(3HO), rather than a copolymer, when grown on sodium octanoate as the sole carbon source. Although this polymer has been produced by other organisms, interestingly this is the first time an absolute homopolymer has been produced by a wild type organism. In addition, a detailed study on the effects of different extraction methods on the yield, molecular weight, thermal properties, and lipopolysaccharide content of P(3HO) has been carried out. The organism was able to accumulate P(3HO) up to 31.38% of its dry cell weight within 48 h in mineral salt medium. Characterization of the monomer was carried out using FTIR, GC-MS, 13C, 1H, and HSQC NMR spectroscopy. The polymer had a crystallinity of 37.5%, Young's modulus value of 11.6 MPa and contact angle of 77.3°. Microstructural studies of solvent cast polymer films revealed a smooth surface topography with a root-mean-square roughness value of 0.238 μm. © 2011 American Chemical Society.

Authors with CRIS profile

Ranjana Rai Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Imperial College London / The Imperial College of Science, Technology and Medicine GB United Kingdom (GB) University of Westminster GB United Kingdom (GB) University of Nottingham GB United Kingdom (GB) University College London (UCL) GB United Kingdom (GB)

How to cite

APA:

Rai, R., Yunos, D.M., Boccaccini, A.R., Knowles, J.C., Barker, I.A., Howdle, S.M.,... Roy, I. (2011). Poly-3-hydroxyoctanoate P(3HO), a Medium Chain Length Polyhydroxyalkanoate Homopolymer from Pseudomonas mendocina. Biomacromolecules, 12, 2126-2136. https://doi.org/10.1021/bm2001999

MLA:

Rai, Ranjana, et al. "Poly-3-hydroxyoctanoate P(3HO), a Medium Chain Length Polyhydroxyalkanoate Homopolymer from Pseudomonas mendocina." Biomacromolecules 12 (2011): 2126-2136.

BibTeX: Download