Development and evaluation of passive autofocus algorithms in the field of automated microscopy
Erpenbeck D, Wittenberg T, Münzenmayer C, Benz M (2014)
Publication Type: Journal article, Original article
Publication year: 2014
Journal
Publisher: Walter de Gruyter
Book Volume: 59
Pages Range: 559-562
Journal Issue: s1
URI: http://www.degruyter.com/view/j/bmte.2014.59.issue-5/issue-files/bmte.2014.59.issue-5.xml
Abstract
This paper presents a comparison of different focus functions applied to microscopic images of blood smears. At the beginning of the article, standard methods for calculating the sharpness of an image are presented. In addition, these methods which are typically based on calculations for grayscale images are extended to the analysis of color images. Furthermore, the influence of calculating these metrics on small "Region of Interests" (ROI) instead of considering the whole image is investigated. For evaluating the results, the focus curves of several focus stacks have been analyzed. Therefore, some criteria for describing the quality of a focus curve are introduced as well. The evaluation shows, that especially in the far focus area the performance of sharpness calculations can be improved by considering color aspects. Furthermore, the choice of selected ROIs is confirmed by the acceleration of these calculations without loss of information.
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Germany (DE)How to cite
APA:
Erpenbeck, D., Wittenberg, T., Münzenmayer, C., & Benz, M. (2014). Development and evaluation of passive autofocus algorithms in the field of automated microscopy. Biomedizinische Technik, 59(s1), 559-562. https://doi.org/10.1515/bmt-2014-5008
MLA:
Erpenbeck, Daniel, et al. "Development and evaluation of passive autofocus algorithms in the field of automated microscopy." Biomedizinische Technik 59.s1 (2014): 559-562.
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