Journal article


Reaction of folic acid with reducing sugars and sugar degradation products


Publication Details
Author(s): Schneider M, Klotzsche M, Werzinger C, Hegele J, Waibel R, Pischetsrieder M
Publisher: American Chemical Society
Publication year: 2002
Volume: 50
Pages range: 1647-1651
ISSN: 0021-8561

Abstract

The reaction of folic acid with reducing sugars (nonenzymatic glycation) under conditions that can occur during food processing and preparation was studied by high-performance liquid chromatography with diode array detection. N-(p-Aminobenzoyl)-L-glutamic acid, a well-established oxidation product, was detected in the reaction mixtures. Furthermore, a new product was isolated and identified as N2-[1-(carboxyethyl)]folic acid (CEF). CEF was the main product that was formed by the nonenzymatic glycation of folic acid. For preparation, N2-[1-(carboxyethyl)]folic acid was obtained in high yields when folic acid and dihydroxyacetone (DHA), a sugar degradation product, were heated at 100°C in phosphate buffer. Mixtures of folic acid and different sugars or DHA were heated under variation of reaction time and temperature, and CEF was quantified. Up to 50% of the vitamin was converted to CEF, with highest yields formed from maltose (49%) and lactose (43%).



How to cite
APA: Schneider, M., Klotzsche, M., Werzinger, C., Hegele, J., Waibel, R., & Pischetsrieder, M. (2002). Reaction of folic acid with reducing sugars and sugar degradation products. Journal of Agricultural and Food Chemistry, 50, 1647-1651. https://dx.doi.org/10.1021/jf011042p

MLA: Schneider, Marc, et al. "Reaction of folic acid with reducing sugars and sugar degradation products." Journal of Agricultural and Food Chemistry 50 (2002): 1647-1651.

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