An ImmunoFET Coupled with an Immunomagnetic Preconcentration Technique for the Sensitive EIS Detection of HF Biomarkers

Ben Halima H, Zine N, Nemeir IA, Pfeiffer N, Heuberger A, Bausells J, Elaissari A, Jaffrezic-Renault N, Errachid A (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Micromachines MDPI

Book Volume: 15

Article Number: 296

Journal Issue: 3

DOI: 10.3390/mi15030296

Abstract

We propose a new strategy using a sandwich approach for the detection of two HF biomarkers: tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10). For this purpose, magnetic nanoparticles (MNPs) (MNPs@aminodextran) were biofunctionalized with monoclonal antibodies (mAbs) using bis (sulfosuccinimidyl) suberate (BS3) as a cross-linker for the pre-concentration of two biomarkers (TNF-α and IL-10). In addition, our ISFETs were biofunctionalized with polyclonal antibodies (pAbs) (TNF-α and IL-10). The biorecognition between pAbs immobilized on the ISFET and the pre-concentrate antigen (Ag) on MNPs was monitored using electrochemical impedance spectroscopy (EIS). Our developed ImmunoFET showed a low detection limit (0.03 pg/mL) toward our target analyte when compared to previously published electrochemical immunosensors. It showed a higher sensitivity than for other HF biomarkers. Finally, the standard addition method was used to determine the unknown concentration in artificial saliva. The results matched with the expected values well.

Authors with CRIS profile

Albert Heuberger Lehrstuhl für Informationstechnik mit dem Schwerpunkt Kommunikationselektronik (Stiftungslehrstuhl) (LIKE)

Additional Organisation(s)

FAU Profilzentrum Licht.Materie.Quantentechnologien (FAU LMQ)

Involved external institutions

Institute of Analytical Sciences / Institut des Sciences Analytiques (ISA) FR France (FR) Fraunhofer-Institut für Integrierte Schaltungen (IIS) DE Germany (DE) Institut de Microelectrònica de Barcelona (IMB-CNM) ES Spain (ES)

How to cite

APA:

Ben Halima, H., Zine, N., Nemeir, I.A., Pfeiffer, N., Heuberger, A., Bausells, J.,... Errachid, A. (2024). An ImmunoFET Coupled with an Immunomagnetic Preconcentration Technique for the Sensitive EIS Detection of HF Biomarkers. Micromachines, 15(3). https://doi.org/10.3390/mi15030296

MLA:

Ben Halima, Hamdi, et al. "An ImmunoFET Coupled with an Immunomagnetic Preconcentration Technique for the Sensitive EIS Detection of HF Biomarkers." Micromachines 15.3 (2024).

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