Reineke B, Paulus I, Löffelsend S, Yu CH, Vinogradov D, Meyer A, Hazur J, Röder J, Vollmer M, Tamgüney G, Hauschild S, Boccaccini AR, Groll J, Förster S (2024)
Publication Type: Journal article
Publication year: 2024
Book Volume: 16
Article Number: 025038
Journal Issue: 2
Bioprinting has evolved into a thriving technology for the fabrication of cell-laden scaffolds. Bioinks are the most critical component for bioprinting. Recently, microgels have been introduced as a very promising bioink, enabling cell protection and the control of the cellular microenvironment. However, the fabrication of the bioinks involves the microfluidic production of the microgels, with a subsequent multistep process to obtain the bioink, which so far has limited its application potential. Here we introduce a direct coupling of microfluidics and 3D-printing for the continuous microfluidic production of microgels with direct in-flow printing into stable scaffolds. The 3D-channel design of the microfluidic chip provides access to different hydrodynamic microdroplet formation regimes to cover a broad range of droplet and microgel diameters. After exiting a microtubing the produced microgels are hydrodynamically jammed into thin microgel filaments for direct 3D-printing into two- and three-dimensional scaffolds. The methodology enables the continuous on-chip encapsulation of cells into monodisperse microdroplets with subsequent in-flow cross-linking to produce cell-laden microgels. The method is demonstrated for different cross-linking methods and cell lines. This advancement will enable a direct coupling of microfluidics and 3D-bioprinting for scaffold fabrication.
APA:
Reineke, B., Paulus, I., Löffelsend, S., Yu, C.H., Vinogradov, D., Meyer, A.,... Förster, S. (2024). On-chip fabrication and in-flow 3D-printing of microgel constructs: from chip to scaffold materials in one integral process. Biofabrication, 16(2). https://doi.org/10.1088/1758-5090/ad3318
MLA:
Reineke, Benjamin, et al. "On-chip fabrication and in-flow 3D-printing of microgel constructs: from chip to scaffold materials in one integral process." Biofabrication 16.2 (2024).
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