Mineral matrix deposition of MC3T3-E1 pre-osteoblastic cells exposed to silicocarnotite and nagelschmidtite bioceramics: In vitro comparison to hydroxyapatite

Rincón-López JA, Hermann-Muñoz JA, Detsch R, Rangel-López R, Muñoz-Saldaña J, Jiménez-Sandoval S, Alvarado-Orozco JM, Boccaccini AR (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Journal of Biomedical Materials Research Part A Wiley

DOI: 10.1002/jbm.a.37699

Abstract

This work presents the effect of the silicocarnotite (SC) and nagelschmidtite (Nagel) phases on in vitro osteogenesis. The known hydroxyapatite of biological origin (BHAp) was used as a standard of osteoconductive characteristics. The evaluation was carried out in conventional and osteogenic media for comparative purposes to assess the osteogenic ability of the bioceramics. First, the effect of the material on cell viability at 24 h, 7 and 14 days of incubation was evaluated. In addition, cell morphology and attachment on dense bioceramic surfaces were observed by fluorescence microscopy. Specifically, alkaline phosphatase (ALP) activity was evaluated as an osteogenic marker of the early stages of bone cell differentiation. Mineralized extracellular matrix was observed by calcium phosphate deposits and extracellular vesicle formation. Furthermore, cell phenotype determination was confirmed by scanning electron microscope. The results provided relevant information on the cell attachment, proliferation, and osteogenic differentiation processes after 7 and 14 days of incubation. Finally, it was demonstrated that SC and Nagel phases promote cell proliferation and differentiation, while the Nagel phase exhibited a superior osteoconductive behavior and could promote MC3T3-E1 cell differentiation to a higher extent than SC and BHAp, which was reflected in a higher number of deposits in a shorter period for both conventional and osteogenic media.

Authors with CRIS profile

Rainer Detsch Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Center for Research and Advanced Studies of the National Polytechnic Institute / Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN) MX Mexico (MX) Universidad Autónoma de Nuevo León MX Mexico (MX) Centro de Ingenieria y Desarollo Industrial (CIDESI) MX Mexico (MX)

How to cite

APA:

Rincón-López, J.A., Hermann-Muñoz, J.A., Detsch, R., Rangel-López, R., Muñoz-Saldaña, J., Jiménez-Sandoval, S.,... Boccaccini, A.R. (2024). Mineral matrix deposition of MC3T3-E1 pre-osteoblastic cells exposed to silicocarnotite and nagelschmidtite bioceramics: In vitro comparison to hydroxyapatite. Journal of Biomedical Materials Research Part A. https://dx.doi.org/10.1002/jbm.a.37699

MLA:

Rincón-López, July Andrea, et al. "Mineral matrix deposition of MC3T3-E1 pre-osteoblastic cells exposed to silicocarnotite and nagelschmidtite bioceramics: In vitro comparison to hydroxyapatite." Journal of Biomedical Materials Research Part A (2024).

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