Physcomitrium patens PpRIC, an ancestral CRIB-domain ROP effector, inhibits auxin-induced differentiation of apical initial cells

Ntefidou M, Eklund DM, le Bail A, Schulmeister S, Scherbel F, Brandl L, Dörfler W, Eichstädt C, Bannmüller A, Ljung K, Kost B (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Cell Reports Elsevier (Cell Press)

Book Volume: 42

Article Number: 112130

Journal Issue: 2

DOI: 10.1016/j.celrep.2023.112130

Abstract

RHO guanosine triphosphatases are important eukaryotic regulators of cell differentiation and behavior. Plant ROP (RHO of plant) family members activate specific, incompletely characterized downstream signaling. The structurally simple land plant Physcomitrium patens is missing homologs of key animal and flowering plant RHO effectors but contains a single CRIB (CDC42/RAC interactive binding)-domain-containing RIC (ROP-interacting CRIB-containing) protein (PpRIC). Protonemal P. patens filaments elongate based on regular division and PpROP-dependent tip growth of apical initial cells, which upon stimulation by the hormone auxin differentiate caulonemal characteristics. PpRIC interacts with active PpROP1, co-localizes with this protein at the plasma membrane at the tip of apical initial cells, and accumulates in the nucleus. Remarkably, PpRIC is not required for tip growth but is targeted to the nucleus to block caulonema differentiation downstream of auxin-controlled gene expression. These observations establish functions of PpRIC in mediating crosstalk between ROP and auxin signaling, which contributes to the maintenance of apical initial cell identity.

Authors with CRIS profile

Maria Ntefidou Lehrstuhl für Zellbiologie Aude le Bail Lehrstuhl für Zellbiologie Sylwia Schulmeister Lehrstuhl für Zellbiologie Franziska Scherbel Lisa Brandl Wolfgang Dörfler Chantal Eichstädt Benedikt Kost Lehrstuhl für Zellbiologie

Involved external institutions

Uppsala University SE Sweden (SE) Umeå Plant Science Centre (UPSC) SE Sweden (SE)

How to cite

APA:

Ntefidou, M., Eklund, D.M., le Bail, A., Schulmeister, S., Scherbel, F., Brandl, L.,... Kost, B. (2023). Physcomitrium patens PpRIC, an ancestral CRIB-domain ROP effector, inhibits auxin-induced differentiation of apical initial cells. Cell Reports, 42(2). https://doi.org/10.1016/j.celrep.2023.112130

MLA:

Ntefidou, Maria, et al. "Physcomitrium patens PpRIC, an ancestral CRIB-domain ROP effector, inhibits auxin-induced differentiation of apical initial cells." Cell Reports 42.2 (2023).

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