Langguth P, Peckert K, Beck P, Kuhnt C, Draßner C, Deinzer A, Steinkasserer A, Wild A (2023)
Publication Type: Journal article
Publication year: 2023
Book Volume: 647
Pages Range: 37-46
DOI: 10.1016/j.bbrc.2023.01.069
Macrophages (MΦ) are remarkably plastic cells, which assume phenotypes in every shade between a pro-inflammatory classical activation, and anti-inflammatory or resolving activation. Therefore, elucidation of mechanisms involved in shaping MΦ plasticity and function is key to understand their role during immunological balance. The immune-modulating CD83 molecule is expressed on activated immune cells and various tissue resident MΦ, rendering it an interesting candidate for affecting MΦ biology. However, in-depth analyses of the precise kinetics and trafficking of CD83 within pro-inflammatory, LPS activated bone-marrow-derived MΦ have not been performed. In this study, we show that activation with LPS leads to a very fast and strong, but transient increase of CD83 expression on these cells. Its expression peaks within 2 h of stimulation and is thereby faster than the early activation antigen CD69. To trace the CD83 trafficking through MΦs, we employed multiple inhibitors, thereby revealing a de novo synthesis and transport of the protein to the cell surface followed by lysosomal degradation, all within 6 h. Moreover, we found a similar expression kinetic and trafficking in human monocyte derived MΦ. This places CD83 at a very early point of MΦ activation suggesting an important role in decisions regarding the subsequent cellular fate.
APA:
Langguth, P., Peckert, K., Beck, P., Kuhnt, C., Draßner, C., Deinzer, A.,... Wild, A. (2023). CD83 acts as immediate early response gene in activated macrophages and exhibits specific intracellular trafficking properties. Biochemical and Biophysical Research Communications, 647, 37-46. https://doi.org/10.1016/j.bbrc.2023.01.069
MLA:
Langguth, Pia, et al. "CD83 acts as immediate early response gene in activated macrophages and exhibits specific intracellular trafficking properties." Biochemical and Biophysical Research Communications 647 (2023): 37-46.
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