Keratinocyte-derived S100A9 modulates neutrophil infiltration and affects psoriasis-like skin and joint disease

Mellor LF, Gago-Lopez N, Bakiri L, Schmidt FN, Busse B, Rauber S, Jimenez M, Megias D, Oterino-Soto S, Sanchez-Prieto R, Grivennikov S, Pu X, Oxford J, Ramming A, Schett G, Wagner EF (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Annals of the Rheumatic Diseases BMJ Publishing Group

DOI: 10.1136/annrheumdis-2022-222229

Abstract

Objectives S100A9, an alarmin that can form calprotectin (CP) heterodimers with S100A8, is mainly produced by keratinocytes and innate immune cells. The contribution of keratinocyte-derived S100A9 to psoriasis (Ps) and psoriatic arthritis (PsA) was evaluated using mouse models, and the potential usefulness of S100A9 as a Ps/PsA biomarker was assessed in patient samples. Methods Conditional S100A9 mice were crossed with DKO* mice, an established psoriasis-like mouse model based on inducible epidermal deletion of c-Jun and JunB to achieve additional epidermal deletion of S100A9 (TKO* mice). Psoriatic skin and joint disease were evaluated in DKO* and TKO* by histology, microCT, RNA and proteomic analyses. Furthermore, S100A9 expression was analysed in skin, serum and synovial fluid samples of patients with Ps and PsA. Results Compared with DKO* littermates, TKO* mice displayed enhanced skin disease severity, PsA incidence and neutrophil infiltration. Altered epidermal expression of selective pro-inflammatory genes and pathways, increased epidermal phosphorylation of STAT3 and higher circulating TNF alpha were observed in TKO* mice. In humans, synovial S100A9 levels were higher than the respective serum levels. Importantly, patients with PsA had significantly higher serum concentrations of S100A9, CP, VEGF, IL-6 and TNF alpha compared with patients with only Ps, but only S100A9 and CP could efficiently discriminate healthy individuals, patients with Ps and patients with PsA. Conclusions Keratinocyte-derived S100A9 plays a regulatory role in psoriatic skin and joint disease. In humans, S100A9/CP is a promising marker that could help in identifying patients with Ps at risk of developing PsA.

Authors with CRIS profile

Simon Rauber Department of Medicine 3 – Rheumatology and Immunology Andreas Ramming Medizinische Fakultät Georg Schett Lehrstuhl für Innere Medizin III

Involved external institutions

Fox Chase Cancer Center US United States (USA) (US) Spanish National Cancer Research Centre / Centro Nacional de Investigaciones Oncológicas (CNIO) ES Spain (ES) Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Universidad de Castilla-La Mancha (UCLM) ES Spain (ES) Boise State University US United States (USA) (US) Medizinische Universität Wien AT Austria (AT)

How to cite

APA:

Mellor, L.F., Gago-Lopez, N., Bakiri, L., Schmidt, F.N., Busse, B., Rauber, S.,... Wagner, E.F. (2022). Keratinocyte-derived S100A9 modulates neutrophil infiltration and affects psoriasis-like skin and joint disease. Annals of the Rheumatic Diseases. https://doi.org/10.1136/annrheumdis-2022-222229

MLA:

Mellor, Liliana F., et al. "Keratinocyte-derived S100A9 modulates neutrophil infiltration and affects psoriasis-like skin and joint disease." Annals of the Rheumatic Diseases (2022).

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