A silicon nitride ISFET based immunosensor for tumor necrosis factor-alpha detection in saliva. A promising tool for heart failure monitoring
Ben Halima H, Bellagambi FG, Alcacer A, Pfeiffer N, Heuberger A, Hangouët M, Zine N, Bausells J, Elaissari A, Errachid A (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 1161
Article Number: 338468
DOI: 10.1016/j.aca.2021.338468
Abstract
According to the European statistics, approximately 26 million patients worldwide suffer from heart failure (HF), and this number seems to be steadily increasing. Inflammation plays a central role in the development of HF, and the pro-inflammatory cytokine Tumor necrosis factor-α (TNF-α) represents inflammation gold-standard biomarker. Early detection plays a crucial role for the prognosis and treatment of HF. An Ion Sensitive Field Effect Transistor (ISFET) based on silicon nitride transducer and biofunctionalized with anti-TNF-α antibody for label-free detection of salivary TNF-α is proposed. Electrochemical impedance spectroscopy (EIS) was used for TNF-α detection. Our ImmunoFET offered a detection limit of 1 pg mL−1, with an analytical reproducibility expressed by a coefficient of variance (CV) resulted < 10% for the analysis of saliva samples, and an analyte recovery of 94 ± 6%. In addition, it demonstrated high selectivity when compared to other HF biomarkers such as Inteleukin-10, N-terminal pro B-type natriuretic peptide, and Cortisol. Finally, ImmunoFET accuracy in determining the unknown concentration of TNF-α was successfully tested in saliva samples by performing standard addition method. The proposed ImmunoFET showed great promise as a complementary tool for biomedical application for HF monitoring by a non-invasive, rapid and accurate assessment of TNF-α.
Authors with CRIS profile
Albert Heuberger
Lehrstuhl für Informationstechnik mit dem Schwerpunkt Kommunikationselektronik (Stiftungslehrstuhl)
Involved external institutions
Université Claude Bernard Lyon 1 (UCB)
France (FR)
Institut de Microelectrònica de Barcelona (IMB-CNM)
Spain (ES)
Fraunhofer-Institut für Integrierte Systeme und Bauelementetechnologie (IISB)
Germany (DE)
National Center for Scientific Research / Centre national de la recherche scientifique (CNRS)
France (FR)
France (FR)
Institut de Microelectrònica de Barcelona (IMB-CNM)
Spain (ES)
Fraunhofer-Institut für Integrierte Systeme und Bauelementetechnologie (IISB)
Germany (DE)
National Center for Scientific Research / Centre national de la recherche scientifique (CNRS)
France (FR)
How to cite
APA:
Ben Halima, H., Bellagambi, F.G., Alcacer, A., Pfeiffer, N., Heuberger, A., Hangouët, M.,... Errachid, A. (2021). A silicon nitride ISFET based immunosensor for tumor necrosis factor-alpha detection in saliva. A promising tool for heart failure monitoring. Analytica Chimica Acta, 1161. https://dx.doi.org/10.1016/j.aca.2021.338468
MLA:
Ben Halima, Hamdi, et al. "A silicon nitride ISFET based immunosensor for tumor necrosis factor-alpha detection in saliva. A promising tool for heart failure monitoring." Analytica Chimica Acta 1161 (2021).
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