Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique

Hazur J, Detsch R, Karakaya E, Kaschta J, Teßmar J, Schneidereit D, Friedrich O, Schubert DW, Boccaccini AR (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Biofabrication IOP Publishing

Book Volume: 12

Article Number: 045004

Journal Issue: 4

DOI: 10.1088/1758-5090/ab98e5

Abstract

Many different biofabrication approaches as well as a variety of bioinks have been developed by researchers working in the field of tissue engineering. A main challenge for bioinks often remains the difficulty to achieve shape fidelity after printing. In order to overcome this issue, a homogeneous pre-crosslinking technique, which is universally applicable to all alginate-based materials, was developed. In this study, the Young's Modulus after post-crosslinking of selected hydrogels, as well as the chemical characterization of alginate in terms of M/G ratio and molecular weight, were determined. With our technique it was possible to markedly enhance the printability of a 2% (w/v) alginate solution, without using a higher polymer content, fillers or support structures. 3D porous scaffolds with a height of around 5 mm were printed. Furthermore, the rheological behavior of different pre-crosslinking degrees was studied. Shear forces on cells as well as the flow profile of the bioink inside the printing nozzle during the process were estimated. A high cell viability of printed NIH/3T3 cells embedded in the novel bioink of more than 85% over a time period of two weeks could be observed.

Authors with CRIS profile

Jonas Hazur Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Rainer Detsch Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Emine Karakaya Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Joachim Kaschta Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Dominik Schneidereit Lehrstuhl für Medizinische Biotechnologie Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie Dirk Schubert Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Julius-Maximilians-Universität Würzburg DE Germany (DE)

How to cite

APA:

Hazur, J., Detsch, R., Karakaya, E., Kaschta, J., Teßmar, J., Schneidereit, D.,... Boccaccini, A.R. (2020). Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique. Biofabrication, 12(4). https://doi.org/10.1088/1758-5090/ab98e5

MLA:

Hazur, Jonas, et al. "Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique." Biofabrication 12.4 (2020).

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