Aflatoxin-specific monoclonal antibody selection for immunoaffinity column development
Ertekin Ö, Kaymak T, Pirinçci ŞŞ, Akçael E, Öztürk S (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 66
Pages Range: 261-268
Journal Issue: 6
Abstract
Antibodies are the basic components of immunoanalytical systems used for detection of a wide range of analytes. Although there are some ground rules for antibody selection, analyte- and assay-specific criteria are the ones that determine the ultimate success of the immunoassays. In this study, we introduced an effective antibody selection procedure for the development of immunoaffinity columns for aflatoxins. The designed scheme puts emphasis on solvent- and matrix-related characterization steps and was used to comparatively evaluate eight monoclonal antibodies. The selected antibody was tolerant to 40% methanol, 20% acetonitrile, 30% acetone and 40% ethanol and did not interact with corn, red pepper or hazelnut extracts. Immunoaffinity columns developed with the selected antibody were validated by 15 independent aflatoxin analysis laboratories.
Authors with CRIS profile
Involved external institutions
General Directorate of Agricultural Research and Policies / Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü (TAGEM)
Turkey (TR)
TÜBİTAK Marmara Research Center / TÜBİTAK Marmara Araştırma Merkezi (TÜBİTAK MAM)
Turkey (TR)
Turkey (TR)
TÜBİTAK Marmara Research Center / TÜBİTAK Marmara Araştırma Merkezi (TÜBİTAK MAM)
Turkey (TR)
How to cite
APA:
Ertekin, Ö., Kaymak, T., Pirinçci, Ş.Ş., Akçael, E., & Öztürk, S. (2019). Aflatoxin-specific monoclonal antibody selection for immunoaffinity column development. Biotechniques, 66(6), 261-268. https://dx.doi.org/10.2144/btn-2018-0143
MLA:
Ertekin, Özlem, et al. "Aflatoxin-specific monoclonal antibody selection for immunoaffinity column development." Biotechniques 66.6 (2019): 261-268.
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