Sauer N (1997)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 1997
Book Volume: 1330
Pages Range: 207-216
Journal Issue: 2
DOI: 10.1016/S0005-2736(97)00169-7
Open Access Link: http://www.sciencedirect.com/science/article/pii/S0005273697001697?via%3Dihub
The activity and the expression of sucrose, hexose and amino acid transporters were studied with flesh, cut or aged tissues and plasma membrane vesicles (PMV) of mature sugar beet (Beta vulgaris L.) leaves. Cutting and ageing both induced an increase of the transcripts coding for sucrose transporters and hexose transporters. NO significant effect could be detected on the amino acid transporter transcripts with the probe used (aap1). A polyclonal serum directed against the Arabidopsis thaliana sucrose transporter (AtSUC1) reacted with a 42 kDa band of the sugar beet PMV, confirming previous biochemical identification of this band as a sucrose transporter. ELISA assays run with microsomal fractions and PMV using the AtSUC1 sucrose transporter probe indicated that ageing, and to a lesser extent cutting, increased the amount of sucrose transporter present in the plasma membrane. However, while cutting strongly stimulated proton-motive force driven uptake of sucrOse in PMV, ageing only resulted in a slight stimulation. These date give evidence for transcriptional, post- transcriptional and post-translational controls of the activity of the sucrose transporter by mechanical treatments. Proton-motive force driven uptake of 3-O-methylglucose and valine in PMV was strongly stimulated in PMV from aged tissues, although previous date had shown that cuffing did not affect theses processes. Therefore, the plant cells possess various levels of control mechanisms that allow them to regulate fluxes of the main assimilates across the plasma membrane when their natural environment is directly or indirectly altered.
APA:
Sauer, N. (1997). Cutting, ageing and expression of plant membrane transporters. Biochimica Et Biophysica Acta-Biomembranes, 1330(2), 207-216. https://dx.doi.org/10.1016/S0005-2736(97)00169-7
MLA:
Sauer, Norbert. "Cutting, ageing and expression of plant membrane transporters." Biochimica Et Biophysica Acta-Biomembranes 1330.2 (1997): 207-216.
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