Identification of the Peptide PyroQ-beta Casein(194-209) as a Highly Specific and Sensitive Marker to Differentiate between Ultrahigh-Temperature Processed (UHT) Milk and Mildly Heated Milk

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autor(en): Dalabasmaz S, Ebner J, Pischetsrieder M
Zeitschrift: Journal of Agricultural and Food Chemistry
Verlag: AMER CHEMICAL SOC
Jahr der Veröffentlichung: 2017
Band: 65
Heftnummer: 49
Seitenbereich: 10781-10791
ISSN: 0021-8561


Abstract


In this study, a new approach was introduced to identify marker peptides that reflect the thermal treatment of commercial milk samples and differentiate ultrahigh-temperature processed (UHT) milk from mildly heated milk. Peptide profiles of training set samples, pasteurized (n = 20), extended shelf life (n = 29), and UHT (n = 29) milk, were recorded by MALDI-TOF-MS after StageTip microextraction. As marker candidates, 13 peptides were selected, and their cutoff levels were defined. The quality of the cutoff levels was then tested with a blind test set. Thus, the peptide m/z 1701.0, which was identified as pyroQ-beta casein(194-209), could ideally differentiate UHT milk from mildly heated milk with an accuracy of 100%. Due to its high reliability and sensitivity, this peptide may be applied in routine analysis to monitor thermal processing of milk. An additional heating experiment showed that the marker peptide candidates are formed during milk processing by endogenous enzymes and selective thermal cleavage.



FAU-Autoren / FAU-Herausgeber

Dalabasmaz, Sevim
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Ebner, Jennifer
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Pischetsrieder, Monika Prof. Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)


Zitierweisen

APA:
Dalabasmaz, S., Ebner, J., & Pischetsrieder, M. (2017). Identification of the Peptide PyroQ-beta Casein(194-209) as a Highly Specific and Sensitive Marker to Differentiate between Ultrahigh-Temperature Processed (UHT) Milk and Mildly Heated Milk. Journal of Agricultural and Food Chemistry, 65(49), 10781-10791. https://dx.doi.org/10.1021/acs.jafc.7b03801

MLA:
Dalabasmaz, Sevim, Jennifer Ebner, and Monika Pischetsrieder. "Identification of the Peptide PyroQ-beta Casein(194-209) as a Highly Specific and Sensitive Marker to Differentiate between Ultrahigh-Temperature Processed (UHT) Milk and Mildly Heated Milk." Journal of Agricultural and Food Chemistry 65.49 (2017): 10781-10791.

BibTeX: 

Zuletzt aktualisiert 2018-03-07 um 15:23