Peukert W, Wanka J (2011)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2011
Book Volume: 34
Pages Range: 510-516
Journal Issue: 4
The second osmotic virial coefficient, B, of protein solutions is a function of the protein interaction potentials and, thus, summarizes many solution parameters leading to crystallization or precipitation of proteins. B of hen egg-white lysozyme and bovine serum albumin was measured by static light scattering under the variation of pH, temperature, and crystallization agents. Comparing B values and precipitate morphology in a systematically analyzed range of solution conditions allows the construction of the so-called crystallization slot. The thermodynamic driving force for crystallization is supersaturation, which is set by the protein concentration and the solubility (related to B). Hence, besides the optimal B range, the protein concentration and, if solubilities are available, the supersaturation leads to a two-dimensional crystallization window where crystal formation is possible. This is shown in a generalized state diagram, where protein morphologies are solely determined as a function of protein concentration or supersaturation and interactions. The second osmotic virial coefficient, B, of protein solutions is a function of the protein interaction potentials. It therefore summarizes many solution parameters leading to crystallization or precipitation of proteins. Values of B were measured for different protein molecules under different solution conditions and were correlated to the results of crystallization experiments. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
APA:
Peukert, W., & Wanka, J. (2011). Optimized production of protein crystals: From 1D crystallization slot towards 2D supersaturation B22 diagram. Chemical Engineering & Technology, 34(4), 510-516. https://doi.org/10.1002/ceat.201000445
MLA:
Peukert, Wolfgang, and Johanna Wanka. "Optimized production of protein crystals: From 1D crystallization slot towards 2D supersaturation B22 diagram." Chemical Engineering & Technology 34.4 (2011): 510-516.
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