Synchronous force and Ca2+ measurements for repeated characterization of excitation-contraction coupling in human myocardium

Sun Z, Lu K, Kamla C, Kameritsch P, Seidel T, Dendorfer A (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Communications Biology Nature Research

Book Volume: 7

Article Number: 220

Journal Issue: 1

DOI: 10.1038/s42003-024-05886-3

Abstract

Dysfunctional Ca2+ signaling affects the myocardial systole and diastole, may trigger arrhythmia and cause transcriptomic and proteomic modifications in heart failure. Thus, synchronous real-time measurement of Ca2+ and force is essential to investigate the relationship between contractility and Ca2+ signaling and the alteration of excitation-contraction coupling (ECC) in human failing myocardium. Here, we present a method for synchronized acquisition of intracellular Ca2+ and contraction force in long-term cultivated slices of human failing myocardium. Synchronous time series of contraction force and intracellular Ca2+ were used to calculate force-calcium loops and to analyze the dynamic alterations of ECC in response to various pacing frequencies, post-pause potentiation, high mechanical preload and pharmacological interventions in human failing myocardium. We provide an approach to simultaneously and repeatedly investigate alterations of contractility and Ca2+ signals in long-term cultured myocardium, which will allow detecting the effects of electrophysiological or pharmacological interventions on human myocardial ECC.

Authors with CRIS profile

Thomas Seidel Professur für Herz-Kreislaufphysiologie

Involved external institutions

Klinikum der Universität München DE Germany (DE)

How to cite

APA:

Sun, Z., Lu, K., Kamla, C., Kameritsch, P., Seidel, T., & Dendorfer, A. (2024). Synchronous force and Ca2+ measurements for repeated characterization of excitation-contraction coupling in human myocardium. Communications Biology, 7(1). https://doi.org/10.1038/s42003-024-05886-3

MLA:

Sun, Zhengwu, et al. "Synchronous force and Ca2+ measurements for repeated characterization of excitation-contraction coupling in human myocardium." Communications Biology 7.1 (2024).

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